       Document 0092
 DOCN  M9630092
 TI    CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against
       Leishmania m. mexicana infection.
 DT    9603
 AU    Lezama-Davila CM; Gallagher G; Centro de Investigaciones en Enfermedades
       Tropicales,; Universidad Autonoma de Campeche, Mexico.
 SO    Mem Inst Oswaldo Cruz. 1995 Jan-Feb;90(1):51-8. Unique Identifier :
       AIDSLINE MED/96043832
 AB    We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG
       anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice
       were either infected with L. m. mexicana promastigotes or vaccinated
       with parasite-membrane antigens incorporated into liposomes.
       Successfully vaccinated mice were used as cell-donors in adoptive
       transfer experiments. Naive, syngeneic recipients received
       highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of
       donors and challenged with live parasites. Our results showed that, both
       CD4+ and CD8+ T cells from infected or vaccinated donors conferred
       significant disease-resistance to naive recipients. In addition,
       adoptive transfer of CD4- CD8- T cells from vaccinated donors
       significantly delayed lesion growth in recipient mice. We concluded that
       vaccination of CBA mice correlates with the induction of protective
       CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG
       anti-Leishmania.
 DE    Animal  *CD4-CD8 Ratio  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY/PARASITOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY/PARASITOLOGY  Electrophoresis, Polyacrylamide
       Gel  Enzyme-Linked Immunosorbent Assay  Female  IgG/ANALYSIS  Leishmania
       mexicana/IMMUNOLOGY  Leishmaniasis, Cutaneous/*IMMUNOLOGY  Male  Mice
       Mice, Inbred CBA  Vaccination  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

