       Document 0087
 DOCN  M9630087
 TI    A delta T-cell receptor deleting element transgenic reporter construct
       is rearranged in alpha beta but not gamma delta T-cell lineages.
 DT    9603
 AU    Shutter J; Cain JA; Ledbetter S; Rogers MD; Hockett RD Jr; Department of
       Medicine, Howard Hughes Medical Institute,; Washington University School
       of Medicine, St. Louis, Missouri; 63110, USA.
 SO    Mol Cell Biol. 1995 Dec;15(12):7022-31. Unique Identifier : AIDSLINE
       MED/96069412
 AB    T cells can be divided into two groups on the basis of the expression of
       either alpha beta or gamma delta T-cell receptors (TCRs). Because the
       TCR delta chain locus lies within the larger TCR alpha chain locus,
       control of the utilization of these two receptors is important in T-cell
       development, specifically for determination of T-cell type:
       rearrangement of the alpha locus results in deletion of the delta coding
       segments and commitment to the alpha beta lineage. In the developing
       thymus, a relative site-specific recombination occurs by which the TCR
       delta chain gene segments are deleted. This deletion removes all D
       delta, J delta, and C delta genes and occurs on both alleles. This delta
       deletional mechanism is evolutionarily conserved between mice and
       humans. Transgenic mice which contain the human delta deleting elements
       and as much internal TCR delta chain coding sequence as possible without
       allowing the formation of a complete delta chain gene were developed.
       Several transgenic lines showing recombinations between deleting
       elements within the transgene were developed. These lines demonstrate
       that utilization of the delta deleting elements occurs in alpha beta T
       cells of the spleen and thymus. These recombinations are rare in the
       gamma delta population, indicating that the machinery for utilization of
       delta deleting elements is functional in alpha beta T cells but absent
       in gamma delta T cells. Furthermore, a discrete population of early
       thymocytes containing delta deleting element recombinations but not V
       alpha-to-J alpha rearrangements has been identified. These data are
       consistent with a model in which delta deletion contributes to the
       implementation of a signal by which the TCR alpha chain locus is
       rearranged and expressed and thus becomes an alpha beta T cell.
 DE    Animal  Base Sequence  Comparative Study  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  DNA Primers  Flow Cytometry  *Gene Deletion
       *Gene Rearrangement, delta-Chain T-Cell Antigen Receptor  Human  Mice
       Mice, Inbred CBA  Mice, Inbred C3H  Mice, Inbred C57BL  Mice, Transgenic
       Molecular Sequence Data  Polymerase Chain Reaction  Receptors, Antigen,
       T-Cell, alpha-beta/*BIOSYNTHESIS/GENETICS  Receptors, Antigen, T-Cell,
       gamma-delta/*BIOSYNTHESIS/GENETICS  Spleen/IMMUNOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte Subsets/*IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY  Thymus Gland/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

