       Document 0076
 DOCN  M9630076
 TI    Isolation of virus-neutralizing RNAs from a large pool of random
       sequences.
 DT    9603
 AU    Pan W; Craven RC; Qiu Q; Wilson CB; Wills JW; Golovine S; Wang JF;
       Department of Biochemistry and Molecular Biology, Pennsylvania; State
       University College of Medicine, Hershey 17033, USA.
 SO    Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11509-13. Unique Identifier
       : AIDSLINE MED/96102143
 AB    RNA and ribonuclease-resistant RNA analogs that bound and neutralized
       Rous sarcoma virus (RSV) were isolated from a large pool of random
       sequences by multiple cycles of in vitro selection using infectious
       viral particles. The selected RNA pool of RSV-binding sequences at a
       concentration of 0.16 microM completely neutralized the virus. Of 19
       sequences cloned from the selected pool, 5 inhibited RSV infection. The
       selected RNA and RNA analogs were shown to neutralize RSV by interacting
       with the virus, rather than by adversely affecting the host cells. The
       selection of the anti-RSV RNA and RNA analogs by intact virions
       immediately suggests the potential application of this approach to
       develop RNA and RNA analogs as inhibitors of other viruses such as human
       immunodeficiency virus.
 DE    Antiviral Agents/*PHARMACOLOGY  Base Sequence  Comparative Study
       Cytidine Triphosphate/ANALOGS & DERIVATIVES/METABOLISM  Gene Library
       Molecular Sequence Data  Oligoribonucleotides/*PHARMACOLOGY
       RNA/*PHARMACOLOGY  Sarcoma Viruses, Avian/*DRUG EFFECTS  Selection
       (Genetics)  Species Specificity  Structure-Activity Relationship
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Transcription,
       Genetic  Uridine Triphosphate/ANALOGS & DERIVATIVES/METABOLISM
       Virion/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

