       Document 0073
 DOCN  M9630073
 TI    Production of avian leukosis virus particles in mammalian cells can be
       mediated by the interaction of the human immunodeficiency virus protein
       Rev and the Rev-responsive element.
 DT    9603
 AU    Nasioulas G; Hughes SH; Felber BK; Whitcomb JM; Gene Expression in
       Eukaryotes Section, National Cancer Institute; Frederick Cancer Research
       and Development Center, Frederick, MD; 21702-1201, USA.
 SO    Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11940-4. Unique Identifier :
       AIDSLINE MED/96102231
 AB    In human immunodeficiency virus type 1-infected cells, the efficient
       expression of viral proteins from unspliced and singly spliced RNAs is
       dependent on two factors: the presence in the cell of the viral protein
       Rev and the presence in the viral RNA of the Rev-responsive element
       (RRE). We show here that the HIV-1 Rev/RRE system can increase the
       expression of avian leukosis virus (ALV) structural proteins in
       mammalian cells (D-17 canine osteosarcoma) and promote the release of
       mature ALV virions from these cells. In this system, the Rev/RRE
       interaction appears to facilitate the export of full-length unspliced
       ALV RNA from the nucleus to the cytoplasm, allowing increased production
       of the ALV structural proteins. Gag protein is produced in the cytoplasm
       of the ALV-transfected cells even in the absence of a Rev/RRE
       interaction. However, a functional Rev/RRE interaction increases the
       amount of Gag present intracellularly and, more strikingly, results in
       the release of mature ALV particles into the supernatant. RCAS virus
       containing an RRE is replication-competent in chicken embryo
       fibroblasts; however, we have been unable to determine whether the
       particles produced in D-17 cells are as infectious as the particles
       produced in chicken embryo fibroblasts.
 DE    Animal  Biological Transport  Blotting, Northern  Cell Compartmentation
       Cell Line  Dogs  Gene Products, rev/*METABOLISM  *Genes, env  *HIV-1
       Leukosis Virus, Avian/*GROWTH & DEVELOPMENT/GENETICS/  ULTRASTRUCTURE
       RNA, Viral/ISOLATION & PURIF  Species Specificity  Support, U.S. Gov't,
       P.H.S.  Viral Structural Proteins/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

