       Document 0072
 DOCN  M9630072
 TI    Immunological response in mice after long-term stimulation with cell
       wall antigens from Brucella melitensis.
 DT    9603
 AU    Galdiero M; Bentivoglio C; Nuzzo I; De Martino L; Molitierno M;
       Carratelli CR; Dipartimento di Patologia, Facolta di Veterinaria,
       Universita; degli Studi di Napoli Federico II, Italy.
 SO    Res Microbiol. 1995 Jul-Aug;146(6):507-15. Unique Identifier : AIDSLINE
       MED/96099691
 AB    The continuous stimulation of the immune system using cell wall antigens
       from Brucella melitensis was found to cause both quantitative and
       qualitative changes in circulating lymphocyte populations in mice.
       Animals were inoculated in the hind legs with antigens on alternate days
       for varying lengths of time. During a two-month period, we saw a higher
       number of circulating lymphocytes, with an increase in the number of
       CD4+ cells (L3T4+) and B lymphocytes (I-Ad). After two months, a drop in
       the overall number of circulating lymphocytes occurred, with a decrease
       in CD4+ cells and an increase in CD8+ cells. During the first two
       months, we observed a size increase in popliteal lymph nodes and an
       elevated humoral response. The response then waned with the declining
       CD4+ cells. In the first two months, the treated animals also showed an
       in vitro response to two mitogens, concanavalin A and lipopolysaccharide
       and to the cell wall fraction, after which the treated animals showed a
       decreased response.
 DE    Animal  Antibody Formation  Antigens, Bacterial/*IMMUNOLOGY
       B-Lymphocytes/*IMMUNOLOGY  Brucella melitensis/*IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Fluorescent Antibody Technique  Hypertrophy/PATHOLOGY  Lymph
       Nodes/IMMUNOLOGY/PATHOLOGY  Lymphocyte Count  Male  Mice  Mice, Inbred
       BALB C  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

