       Document 0071
 DOCN  M9630071
 TI    Expression and quantification of IgG and IgM molecules on the surface of
       lymphocytes of cattle infected with bovine leukaemia virus.
 DT    9603
 AU    Levkut M; Ponti W; Soligo D; Quirici N; Rocchi M; Lambertenghi Deliliers
       G; Department of Pathological Anatomy, University of Veterinary;
       Medicine, Kosice, Slovakia.
 SO    Res Vet Sci. 1995 Jul;59(1):45-9. Unique Identifier : AIDSLINE
       MED/96131126
 AB    Immunogold-labelled antibodies were used with scanning electron
       microscopy (SEM) and fluorescent-labelled antibodies were used with flow
       cytometry (FACS) to evaluate the expression and quantity of IgG and IgM
       molecules on the surface of the lymphocytes of cattle infected with
       bovine leukaemia virus (BLV). The BLV-infected animals were divided
       serologically and haematologically into groups with (BLV+PL+) and
       without (BLV+PL-) persistent lymphocytosis (PL). The percentage of
       IgM-bearing cells was significantly higher in the BLV+PL+ group than in
       the BLV-PL- and BLV+PL- groups by FACS. There was a significantly higher
       percentage of IgG-bearing cells in the BLV+PL+ group than in the BLV-PL-
       and BLV+PL- groups by SEM, but no differences were found by FACS. A
       significantly higher intensity of IgM expression was observed in the
       BLV+PL+ group by SEM. A higher intensity of IgG expression in some
       animals was detected only by SEM. An increase in the number of larger
       IgM cells were observed in the BLV+PL- and BLV+PL+ groups by SEM. The
       SEM analysis was more sensitive than FACS in this experiment.
 DE    Animal  Antibodies, Monoclonal  Antibodies, Viral/IMMUNOLOGY/ISOLATION &
       PURIF  Antigens, Surface/*BIOSYNTHESIS  Cattle  Enzootic Bovine
       Leukosis/*IMMUNOLOGY  Flow Cytometry/VETERINARY  IgG/*BIOSYNTHESIS
       IgM/*BIOSYNTHESIS  Lymphocytes/*IMMUNOLOGY  Microscopy, Electron,
       Scanning/VETERINARY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

