       Document 0064
 DOCN  M9630064
 TI    [Detection of fecal Cryptosporidium parvum antigens using an ELISA
       technique]
 DT    9603
 AU    Weitz JC; Departamento de Medicina, Facultad de Medicina, Universidad
       de; Chile (Campus Occidente), Santiago de Chile.
 SO    Rev Med Chil. 1995 Mar;123(3):330-3. Unique Identifier : AIDSLINE
       MED/96041714
 AB    ELISA techniques using monoclonal antibodies have been recently
       developed for the detection of Cryptosporidium parvum fecal antigens.
       The aim of this work was to compare the yield of these ELISA techniques
       with Ziehl-Nielsen and Safranin stains in formaldehyde-salt fixed
       samples. One hundred five fecal samples of patients with acute diarrhea
       were studied. Forty-seven samples did not contain Cryptosporidium and
       ELISA was negative. Also, ELISA was positive in 52 or 58 samples that
       contained Cryptosporidium (89.6%); the samples with false negative
       results contained scanty oocysts. Seven intensely positive Paf-fixed
       stool samples from AIDS patients with chronic diarrhea, were not
       reactive to ELISA. There was a good correlation between visually and
       mechanically read samples and there were no false positives. It is
       concluded that ELISA cannot be used in Paf-fixed samples and has a lower
       sensitivity that the stained commonly used for the diagnosis of
       cryptosporidiosis.
 DE    Animal  Antigens, Protozoan/*ANALYSIS  Child  Comparative Study
       Cryptosporidiosis/*DIAGNOSIS  Cryptosporidium parvum/*IMMUNOLOGY
       English Abstract  Enzyme-Linked Immunosorbent Assay  Feces/*PARASITOLOGY
       Human  Sensitivity and Specificity  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

