       Document 0055
 DOCN  M9630055
 TI    Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major
       HIV-suppressive factors produced by CD8+ T cells.
 DT    9603
 AU    Cocchi F; DeVico AL; Garzino-Demo A; Arya SK; Gallo RC; Lusso P;
       Laboratory of Tumor Cell Biology, National Cancer Institute,; Bethesda,
       MD 20892, USA.
 SO    Science. 1995 Dec 15;270(5243):1811-5. Unique Identifier : AIDSLINE
       MED/96106406
 AB    Evidence suggests that CD8+ T lymphocytes are involved in the control of
       human immunodeficiency virus (HIV) infection in vivo, either by
       cytolytic mechanisms or by the release of HIV-suppressive factors
       (HIV-SF). The chemokines RANTES, MIP-1 alpha, and MIP-1 beta were
       identified as the major HIV-SF produced by CD8+ T cells. Two active
       proteins purified from the culture supernatant of an immortalized CD8+ T
       cell clone revealed sequence identity with human RANTES and MIP-1 alpha.
       RANTES, MIP-1 alpha, and MIP-1 beta were released by both immortalized
       and primary CD8+ T cells. HIV-SF activity produced by these cells was
       completely blocked by a combination of neutralizing antibodies against
       RANTES, MIP-1 alpha, and MIP-1 beta. Recombinant human RANTES, MIP-1
       alpha, and MIP-1 beta induced a dose-dependent inhibition of different
       strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). These
       data may have relevance for the prevention and therapy of AIDS.
 DE    Adult  Amino Acid Sequence  Animal  Cell Division/PHYSIOLOGY  Cell Line
       Cells, Cultured  Culture Media, Conditioned  Cytokines/ANTAGONISTS &
       INHIB/*IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Dose-Response
       Relationship, Immunologic  Escherichia coli  Herpesvirus 6,
       Human/IMMUNOLOGY  Herpesvirus 7, Human/IMMUNOLOGY  Human  HIV
       Infections/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  HIV-2/IMMUNOLOGY
       HTLV-I/IMMUNOLOGY  IgG/IMMUNOLOGY  Lymphocyte Transformation  Macaca
       nemestrina  Molecular Sequence Data  Monokines/ANTAGONISTS &
       INHIB/*IMMUNOLOGY  Recombinant Proteins/IMMUNOLOGY  RANTES/ANTAGONISTS &
       INHIB/*IMMUNOLOGY  SIV/IMMUNOLOGY  Virus Inhibitors/*PHYSIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

