       Document 0050
 DOCN  M9630050
 TI    Effects of cytokines on HIV-1 production by thymocytes.
 DT    9603
 AU    Uittenbogaart CH; Anisman DJ; Zack JA; Economides A; Schmid I; Hays EF;
       Department of Pediatrics, UCLA School of Medicine, USA.
 SO    Thymus. 1994-95;23(3-4):155-75. Unique Identifier : AIDSLINE
       MED/96076591
 AB    The thymus is essential for normal T cell development and is
       particularly active during fetal and postnatal life. Here we describe in
       vitro studies of HIV-infected thymocytes cultured with cytokines
       normally produced in the thymus. Virus expression was determined by
       measuring p24 antigen levels in the culture supernatants. Addition of
       IL-2+IL-4 and IL-4+IL-7 to the HIV-infected cultures of both fetal and
       postnatal thymocytes resulted in various levels of synergistic
       expression of p24 antigen. When differences in phenotype between
       HIV-infected and non-infected (sham-treated) cultures from the same
       specimen were evaluated, there was a decrease in the percentages and
       absolute numbers of CD4-bearing cells in HIV-infected thymocytes
       cultured with IL-2+IL-4. Studies were done to determine if synergy in
       HIV expression was mediated by activation, proliferation or induction or
       suppression of other cytokines. We found a higher percentage of
       activated CD4+CD8+/high cells in thymocytes cultured with IL-2+IL-4 and
       IL-4+IL-7 than in thymocytes cultured with IL-2+IL-7. Proliferation was
       higher in thymocytes cultured with cytokine combinations but did not
       correlate with those conditions showing synergy. IL-4 reduced IFN-gamma
       production by thymocytes cultured with IL-2 in both HIV-infected and
       non-infected thymocytes. In addition, exogenous IFN-gamma decreased p24
       expression by HIV-infected thymocytes when cultured with IL-4 alone,
       with IL-2+IL-4 or IL-4+IL-7. These results suggest that suppression of
       IFN-gamma by IL-4 may combine with cell activation and proliferation to
       produce synergy of virus expression observed with IL-2+IL-4 and
       IL-4+IL-7.
 DE    Aging/IMMUNOLOGY  Cells, Cultured  Child  Cytokines/*PHARMACOLOGY
       Dactinomycin/ANALOGS & DERIVATIVES/PHARMACOLOGY  Fetal
       Development/IMMUNOLOGY  Fetus  Gestational Age  Granulocyte-Macrophage
       Colony-Stimulating Factor/PHARMACOLOGY  Human  HIV Core Protein
       p24/ANALYSIS/BIOSYNTHESIS  HIV-1/DRUG EFFECTS/*PHYSIOLOGY
       Interferon-gamma, Recombinant/PHARMACOLOGY  Interleukin-1/PHARMACOLOGY
       Interleukin-4/PHARMACOLOGY  Interleukin-6/PHARMACOLOGY
       Interleukin-7/PHARMACOLOGY  Interleukins/*PHARMACOLOGY  Kinetics
       Lymphocyte Transformation/DRUG EFFECTS  Recombinant
       Proteins/PHARMACOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  T-Lymphocytes/DRUG EFFECTS/IMMUNOLOGY/*VIROLOGY  Tumor Necrosis
       Factor/PHARMACOLOGY  Virus Replication/DRUG EFFECTS/*PHYSIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

