       Document 0043
 DOCN  M9630043
 TI    Assembly of SIV virus-like particles containing envelope proteins using
       a baculovirus expression system.
 DT    9603
 AU    Yamshchikov GV; Ritter GD; Vey M; Compans RW; Department of Microbiology
       and Immunology, Emory University; School of Medicine, Atlanta, Georgia
       30322, USA.
 SO    Virology. 1995 Dec 1;214(1):50-8. Unique Identifier : AIDSLINE
       MED/96095223
 AB    The requirements for SIV particle assembly and envelope incorporation
       were investigated using a baculovirus expression system. The Pr56gag
       precursor protein expressed under control of the polyhedrin promoter
       (pPolh) produced high levels of immature retrovirus-like particles (VLP)
       upon expression in Sf9 insect cells. To determine the optimal conditions
       for envelope protein (Env) incorporation into VLP, two recombinant
       baculoviruses expressing the SIV envelope protein under control of a
       very late pPolh or a hybrid late/very late capsid/polyhedrin (Pcap/polh)
       promoter and a recombinant expressing a truncated form of the SIV
       envelope protein (Envt) under the hybrid Pcap/polh promoter were
       compared. We have observed that utilization of the earlier hybrid
       promoter resulted in higher levels of Env expression on the cell surface
       and its incorporation into budding virus particles. We have also found
       that the Envt protein is transported to the cell surface of insect cells
       and incorporated into VLP more efficiently than full-length Env. In
       addition, we examined the effect of coexpression of the protease furin,
       which has been implicated in the proteolytic cleavage of the Env
       precursor gp160 in mammalian cells. Coexpression of furin in insect
       cells resulted in more efficient proteolytic cleavage into gp120 and
       gp41, and the cleaved proteins were incorporated into VLP.
 DE    Animal  Base Sequence  Cell Line  DNA Primers  Gene Products,
       env/METABOLISM  Gene Products, gag/GENETICS/METABOLISM  Genetic Vectors
       HIV Envelope Protein gp120/METABOLISM  Molecular Sequence Data  Nuclear
       Polyhedrosis Virus/GENETICS  Protein Precursors/METABOLISM  Spodoptera
       Subtilisins/METABOLISM  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  SIV/*PHYSIOLOGY/ULTRASTRUCTURE  Viral Envelope
       Proteins/GENETICS/*METABOLISM  Virion/*PHYSIOLOGY  *Virus Assembly
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

