       Document 0042
 DOCN  M9630042
 TI    VP1 and VP2 capsid proteins of Theiler's virus are targets of
       H-2D-restricted cytotoxic lymphocytes in the central nervous system of
       B10 mice.
 DT    9603
 AU    Lin X; Thiemann NR; Pease LR; Rodriguez M; Department of Immunology,
       Mayo Clinic, Rochester, Minnesota; 55905, USA.
 SO    Virology. 1995 Dec 1;214(1):91-9. Unique Identifier : AIDSLINE
       MED/96095227
 AB    Resistance to Theiler's virus-induced demyelination maps genetically to
       the MHC class I D region and is associated with up-regulation of class I
       products and the presence of MHC-restricted virus-specific cytotoxic
       CD8+ T cells in the CNS. To determine the targets of the cytotoxic
       response, transfected C57SV (Kb, Db) cells expressing LP (including the
       leader peptide, VP4, VP2, and VP3 coding sequences), VP4 (including the
       leader peptide and VP4), VP2, VP3, VP1, or RP (including P2 and P3) were
       generated. CNS-infiltrating lymphocytes obtained from virus-infected
       B10, B10.K (Kk, Dk), B10.RBF (Kb, Df). B10.RFB3 (Kf, Db), and B10.RBQ
       (Kb, Dq) mice were used as effectors. Specific cytotoxicity to the
       capsid proteins encoded in the LP construct, VP2 and VP1, was
       demonstrated to be H-2Db region restricted and was mediated by CD8+ T
       cells. No Kb-restricted virus-specific cytotoxicity response was
       observed. No specific cytotoxic response against RP-encoded proteins was
       observed in the CNS of B10 mice. Therefore, both VP1 and VP2 are targets
       for an H-2D-restricted cytotoxic immune response against Theiler's virus
       infection in the CNS of infected resistant B10 mice.
 DE    Animal  Base Sequence  Capsid/GENETICS/*IMMUNOLOGY  Cell Line  Central
       Nervous System/CYTOLOGY/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  DNA, Viral  H-2 Antigens  Mice  Molecular
       Sequence Data  Phenotype  Poliomyelitis/*IMMUNOLOGY/VIROLOGY
       Polioviruses, Murine/GENETICS/*IMMUNOLOGY/METABOLISM  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

