       Document 0033
 DOCN  M9630033
 TI    Genital-associated lymphoid tissue in female non-human primates.
 DT    9603
 AU    Lehner T; Panagiotidi C; Bergmeier LA; Tao L; Brookes R; Gearing A;
       Adams S; Department of Immunology, UMDS of Guy's Hospital, London,
       United; Kingdom.
 SO    Adv Exp Med Biol. 1995;371A:357-65. Unique Identifier : AIDSLINE
       MED/96001537
 AB    We investigated genital-associated lymphoid tissue (GENALT) in non-human
       primates (macaques), by augmenting vaginal with oral immunization. The
       vaccine was a recombinant particulate SIV antigen (p27:Ty-VLP), linked
       to CT-B, and administered into the vagina by a paediatric naso-gastric
       tube and into the stomach by a gastric tube. Oro-vaginal or vagino-oral
       sequence of immunization elicited specific CD4+ T cell proliferative
       responses to p27 antigen in the genital lymph nodes and the spleen but
       not in unrelated lymph nodes. CD4+ T cells reconstituted with B cells
       and macrophages from the genital lymph nodes induced specific IgA and to
       a lesser extent IgG anti-p27 antibodies. However, the corresponding
       splenic cells induced greater IgG than IgA antibody synthesis.
       Intramuscular immunization primed splenic but not genital lymph node
       cells, and induced CD4+ T cell proliferative responses and predominantly
       B cell IgG antibody synthesis. Finding primed B and T cells in the
       genital lymph nodes after augmenting vaginal by oral immunization
       provides experimental evidence for GENALT in non-human primates. This
       primate model of vaginal immunization suggests 3 levels of specific
       immunity: (1) secretory IgA (and IgG) in the cervico-vaginal mucosal
       epithelium; (2) primed CD4+ T cells and B cells in the genital lymph
       nodes and the spleen; and (3) circulating CD4+ T cells, B cells and IgG
       and IgA antibodies specific to the immunizing antigen.
 DE    Administration, Intravaginal  Administration, Oral  Animal  Antibody
       Formation  Antibody Specificity  Cervix Uteri/*IMMUNOLOGY  Cholera
       Toxin/ADMINISTRATION & DOSAGE/GENETICS/IMMUNOLOGY  Female  Gene
       Products, gag/ADMINISTRATION & DOSAGE/GENETICS/*IMMUNOLOGY
       IgA/BIOSYNTHESIS  IgG/BIOSYNTHESIS  Lymph Nodes/IMMUNOLOGY  Lymphocyte
       Cooperation  Lymphocyte Subsets/*IMMUNOLOGY  Lymphoid Tissue/*IMMUNOLOGY
       Macaca fascicularis/*IMMUNOLOGY  Macaca mulatta/*IMMUNOLOGY
       Macrophages/IMMUNOLOGY  Retrotransposons/GENETICS  Spleen/IMMUNOLOGY
       SIV/GENETICS/*IMMUNOLOGY  Vaccination/*METHODS  Vaccines,
       Synthetic/*ADMINISTRATION & DOSAGE/IMMUNOLOGY  Vagina/*IMMUNOLOGY
       Vibrio cholerae/IMMUNOLOGY  Viral Vaccines/*ADMINISTRATION &
       DOSAGE/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

