       Document 0026
 DOCN  M9630026
 TI    Dendritic cells as stimulator cells of MHC class I-restricted immune
       responses.
 DT    9603
 AU    Elbe A; Stingl G; Department of Dermatology, Univ. of Vienna Medical
       School, VIRCC,; Austria.
 SO    Adv Exp Med Biol. 1995;378:341-5. Unique Identifier : AIDSLINE
       MED/96036873
 AB    We have shown that growth factor-dependent, MHC class I+/II dendritic
       cell lines established from mouse fetal skin, can stimulate naive,
       allogeneic but not syngeneic CD8+ T cells in the absence of CD4+ T cells
       and that this T cell response is restricted by MHC class I molecules. We
       further showed that the FSCL-induced activation of naive CD8+ T cells is
       critically dependent on the physical contact between stimulator and
       responder cells and the expression of the costimulatory molecule B7 on
       FSCL. An important question that remains to be addressed concerns the
       derivation of FSCL. One could argue that they are members of the LC/DC
       family because they (i) exhibit certain features of fetal murine LC
       (i.e., CD45+, CD44+, CD32+, MHC class I+, MHC class II-, asialo GM1+,
       TCR-) including membrane-bound ADPase activity (A. Elbe, unpublished
       observation) and (ii) exhibit a pronounced dendritic configuration when
       cultured. If these cells are indeed derived from fetal LC, they should
       undergo the same phenotypic changes (MHC class II(-)-->MHC class II+)
       under in vitro culture conditions as do fetal LC in situ. However, our
       FSCL are phenotypically stable, and attempts to induce MHC class II
       expression with cytokine cocktails were unsuccessful. One explanation
       for this phenomenon could be that stimulatory signals provided by fetal
       keratinocytes or other skin cells are responsible for LC maturation in
       vivo and that, due to the early demise of these stromal cells in fetal
       skin cell cultures, the maturation process would not have been
       completed.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Cell Communication  Cell Line  Cytokines/METABOLISM
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Dendritic Cells/*IMMUNOLOGY  Fetus
       Histocompatibility Antigens Class I/*METABOLISM  Lymphocyte
       Transformation  Mice  Skin/CYTOLOGY/IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

