       Document 0015
 DOCN  M9630015
 TI    Cell-cell interactions regulate dendritic cell-dependent HIV-1
       production in CD4+ T lymphocytes.
 DT    9603
 AU    Pinchuk LM; Polacino PS; Agy MB; Klaus SJ; Clark EA; Regional Primate
       Research Center, University of Washington; Medical Center, Seattle
       98195, USA.
 SO    Adv Exp Med Biol. 1995;378:461-3. Unique Identifier : AIDSLINE
       MED/96036900
 AB    We investigated the role of blood dendritic cells (DC) in transmission
       of HIV-1 from infected to uninfected CD4+ T cells, and the accessory
       molecules involved. DC promoted transmission from infected to uninfected
       CD4+ cells, but blood DC themselves were not infectable. DC-mediated
       transmission was blocked by mAb to CD4 and MHC class II, but strongly
       increased by mAb to CD40 on DC or CD28 on T cells. The DC-dependent
       infection was inhibitable by anti-CD80 and a soluble fusion protein of
       the CD80 ligand, CTLA4; soluble CTLA4Ig also blocked infection augmented
       by crosslinking CD40. We also demonstrated that mAb to CD40 up-regulate
       the expression of CTLA4 ligands CD80 and B70/B7-2 (CD86) on DC. These
       data suggest that the dialog between CD40-CD40 ligand (CD40L) and
       CD28-CD80 counter-receptors on DC and T cells may be linked to HIV
       infection in vivo.
 DE    Antibodies, Blocking/PHARMACOLOGY  Antibodies, Monoclonal/PHARMACOLOGY
       Antigens, CD/METABOLISM  Antigens, CD28/METABOLISM  Antigens,
       CD40/METABOLISM  Antigens, CD80/METABOLISM  Antigens,
       Differentiation/METABOLISM  Blood Cells/IMMUNOLOGY/VIROLOGY  Cell
       Communication  CD4-Positive T-Lymphocytes/*IMMUNOLOGY/*VIROLOGY
       Dendritic Cells/*IMMUNOLOGY/*VIROLOGY  Human  HIV
       Infections/IMMUNOLOGY/VIROLOGY  HIV-1/PHYSIOLOGY/*PATHOGENICITY  In
       Vitro  Ligands  Membrane Glycoproteins/METABOLISM  Up-Regulation
       (Physiology)  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

