       Document 0012
 DOCN  M9630012
 TI    Infection of cultured immature dendritic cells with human
       immunodeficiency virus type 1.
 DT    9603
 AU    Hausser GA; Hultgren C; Akagawa K; Tsunetsugu-Yokota Y; Meyerhans A;
       Abteilung Virologie, Institut fur Medizinische Mikrobiologie und;
       Hygiene, Freiburg, Germany.
 SO    Adv Exp Med Biol. 1995;378:477-9. Unique Identifier : AIDSLINE
       MED/96036904
 AB    An in vitro culture system was developed that facilitates detailed
       studies of the interaction of Human Immunodeficiency Virus (HIV) with
       dendritic cells (DC). Cultured immature DC were generated from adherent
       peripheral blood mononuclear cells in the presence of GM-CSF and IL-4.
       These cells were non-adherent, non-phagocytic and had a veiled surface
       appearance. They expressed high levels of MHC class I and II proteins,
       CD1a, B7/BB1 and low levels of CD4, and were known to possess a potent
       soluble antigen presenting capacity. Upon infection with the HIV-1
       strains Lai (lymphocytotropic) and BaL (monocytotropic), the viral RNA
       was reverse transcribed to complete DNA provirus. However the infection
       was non-productive as judged from measuring the activity of the virus
       encoded reverse transcriptase in the culture supernatant. Thus HIV
       infection was restricted at a step post entry.
 DE    Antigen Presentation  Antigens, Surface/METABOLISM  Cell Differentiation
       Cells, Cultured  Dendritic Cells/CYTOLOGY/IMMUNOLOGY/*VIROLOGY  Human
       HIV Infections/ETIOLOGY  HIV-1/GENETICS/PHYSIOLOGY/*PATHOGENICITY  In
       Vitro  Proviruses/GENETICS/PHYSIOLOGY  Support, Non-U.S. Gov't  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

