       Document 1083
 DOCN  M9621083
 TI    Enhancement of CTL response induced by a viral peptide using cationized
       BSA, a Th1-stimulating adjuvant.
 DT    9602
 AU    Vasilakos JP; Michael JG; Department of Molecular Genetics,
       Biochemistry, and Microbiology,; University of Cincinnati, Ohio
       45267-0524, USA.
 SO    Viral Immunol. 1994;7(4):179-88. Unique Identifier : AIDSLINE
       MED/96100712
 AB    We have recently shown that it is possible to activate cytotoxic T cells
       (CTL) in vivo with HSV-1 glycoprotein B H-2Kb-restricted peptide (gB
       peptide) independent of CD4+ T cell help. Here we report that the gB
       peptide-specific CTL response is significantly enhanced when mice are
       immunized with a mixture of gB peptide and cationized BSA (cBSA). The
       latter molecule is a positively charged form of the native BSA molecule
       that stimulates CD4+ T cells to produce cytokines characteristic of Th1
       cells. The cBSA-enhanced CTL response required the presence of CD4+ T
       cells, but it did not require stimulation in vitro by antigen or
       exogenous cytokines. gB peptide/cBSA-activated LN cells transcribed IL-2
       and IFN-gamma, but only IL-2 was essential for CTL development. Our data
       demonstrate that while activation of CTL may occur in the absence of
       CD4+ cells, cytokines produced by CD4+ Th1 cells provide stimulatory
       signals during CTL maturation. Thus, cotreatment with a substance that
       activates Th1 CD4+ cells may be useful for achieving maximal CTL
       responsiveness.
 DE    Adjuvants, Immunologic/*PHARMACOLOGY  Animal  Cations  Cell
       Differentiation/IMMUNOLOGY  Cytotoxicity, Immunologic/*DRUG EFFECTS
       Female  Interferon Type II/PHYSIOLOGY
       Interleukin-2/BIOSYNTHESIS/PHYSIOLOGY  Mice  Mice, Inbred C57BL  Serum
       Albumin, Bovine/CHEMISTRY/IMMUNOLOGY/*PHARMACOLOGY
       Simplexvirus/*IMMUNOLOGY  T-Lymphocytes, Cytotoxic/DRUG
       EFFECTS/*IMMUNOLOGY  Th1 Cells/*CHEMISTRY/METABOLISM  Viral Envelope
       Proteins/IMMUNOLOGY/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

