       Document 1073
 DOCN  M9621073
 TI    Determination of HIV-1 subtypes in injecting drug users in Bangkok,
       Thailand, using peptide-binding enzyme immunoassay and heteroduplex
       mobility assay: evidence of increasing infection with HIV-1 subtype E.
 DT    9602
 AU    Wasi C; Herring B; Raktham S; Vanichseni S; Mastro TD; Young NL;
       Rubsamen-Waigmann H; von Briesen H; Kalish ML; Luo CC; et al; Department
       of Microbiology, Faculty of Medicine, Siriraj; Hospital, Mahidol
       University, Bangkok, Thailand.
 SO    AIDS. 1995 Aug;9(8):843-9. Unique Identifier : AIDSLINE MED/96014956
 AB    OBJECTIVES: To evaluate the sensitivity and specificity of
       peptide-binding enzyme immunoassay (PEIA) and heteroduplex mobility
       assay (HMA) for the determination of HIV-1 subtypes B and E; to
       determine the proportions of infections due to subtypes B and E over
       time; and to generate data on DNA sequences of the C2-V3 region of the
       env genes. METHODS: HIV-1 subtyping was conducted by PEIA and HMA on
       blood specimens obtained from 97 injecting drug users (IDU) infected
       with HIV between 1988 and 1993. Genetic sequencing was performed on 84
       specimens. RESULTS: Both laboratory methods were highly sensitive and
       specific for the determination of HIV-1 subtypes B and E. The two tests
       were complementary; samples which could not be typed by HMA were
       correctly typed by PEIA and vice versa. While subtype B accounted for
       80.4% (78 out of 97) of infections overall, the proportion of new
       infections due to subtype E increased from 2.6% (one out of 38) in
       1988-1989 to 25.6% (11 out of 43) in 1990-1991, and to 43.8% (seven out
       of 16) in 1992-1993 (chi 2 for linear trend, P < 0.001). CONCLUSIONS:
       HMA and PEIA are practical, sensitive and specific laboratory methods
       for the determination of HIV-1 subtypes in Thailand, and may be useful
       in other geographic areas to define the molecular epidemiology of the
       global HIV-1 pandemic. Data suggest that the proportion subtype E
       infections have increased among Bangkok IDU from 1988 through 1993.
 DE    Adult  Amino Acid Sequence  Epidemiology, Molecular  Evaluation Studies
       Female  Genes, env  Human  HIV
       Infections/*COMPLICATIONS/EPIDEMIOLOGY/*VIROLOGY
       HIV-1/*CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Immunoenzyme
       Techniques/STATISTICS & NUMER DATA  Male  Molecular Sequence Data
       Peptides/GENETICS  Sensitivity and Specificity  Substance Abuse,
       Intravenous/*COMPLICATIONS/*VIROLOGY  Thailand/EPIDEMIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

