       Document 1037
 DOCN  M9621037
 TI    Characterization of V3 loop-binding protein(s) of Molt-4 and U937 cells.
 DT    9602
 AU    Xu Y; Murakami T; Kawase S; Uchiyama T; Hattori T; Laboratory for AIDS
       Immunology, Kyoto University, Japan.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):563-70. Unique Identifier :
       AIDSLINE MED/96093891
 AB    The V3 loop in gp120 of human immunodeficiency virus type 1 (HIV-1) is
       known as a principal neutralizing and cell-tropic determinant.
       Biotinylated synthetic V3 loop peptides derived from three different
       HIV-1 strains were used as ligands to identify the cell surface
       counterreceptor, which may participate in the infection of HIV-1. Two
       different cell lines, Molt-4 and U937, and three V3 loop peptides
       derived from LAVELI, HTLV-IIIMN, and HTLV-IIIB strains were used. The
       binding of HTLV-IIIB-derived peptide to the cell surface was confirmed
       using 125I-labeled surface proteins of both cell lines. The relative
       molecular mass of the major radioactive band on the autoradiogram was
       32-33 kDa in both cell lines. A protein was purified from the plasma
       membrane fraction of Molt-4 cells using affinity columns coupled with
       three different V3 loop peptides. Two major polypeptides (32 and 33 kDa)
       were eluted from the affinity column. Size-exclusion chromatography
       showed that the protein migrated as a single peak with a molecular mass
       of 130 kDa. These proteins were separated by reversed-phase
       chromatography, which indicated that the 32-kDa protein is more
       hydrophobic than the 33-kDa protein in Molt-4 cells. A similar but not
       identical 130-kDa protein with 32- and 33-kDa polypeptides were also
       purified from U937 cells. These findings indicate that HIV-1 utilizes a
       tetrameric protein on the surface of Molt-4 and U937 cells on infection.
 DE    Amino Acid Sequence  Chromatography, Affinity  Chromatography, High
       Pressure Liquid  CD4-Positive T-Lymphocytes/METABOLISM/VIROLOGY  Human
       HIV Envelope Protein gp120/*METABOLISM  HIV-1/*METABOLISM  Molecular
       Sequence Data  Peptide Fragments/*METABOLISM  Protein Binding
       Receptors, HIV/ISOLATION & PURIF/*METABOLISM  Support, Non-U.S. Gov't
       Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

