       Document 1032
 DOCN  M9621032
 TI    Molecular characterization of human immunodeficiency virus type 1
       isolates from Venezuela.
 DT    9602
 AU    Quinones-Mateu ME; Dopazo J; Este JA; Rota TR; Domingo E; Centro de
       Biologia Molecular Severo Ochoa (CSIC-UAM),; Universidad Autonoma de
       Madrid, Cantoblanco, Spain.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):605-16. Unique Identifier :
       AIDSLINE GENBANK/U16779
 AB    Eight HIV-1 isolates from Venezuela have been characterized by
       nucleotide sequencing of the entire reverse transcriptase (RT)- and
       surface glycoprotein (gp 120)-coding regions. Average mutant frequencies
       were 2.5 x 10(-2) substitutions per nucleotide (s/nt) for the RT-coding
       region, and 10 x 10(-2) or 6.8 x 10(-2) s/nt for the gp120-coding
       region, depending on whether gaps introduced for optimal alignment were
       or were not, respectively, considered in the calculations. Phylogenetic
       trees were derived by maximum-likelihood, neighbor-joining, and maximum
       parsimony methods. In the trees derived from both RT- and gp120-coding
       regions, Venezuelan isolates cluster with subtype B viruses. However,
       the relative position of some of the isolates is considerably different
       in the two trees. Unique V3 loop amino acid sequences, not represented
       in the current database, have been identified among the Venezuelan
       isolates. In addition to representing the first molecular
       characterization of HIV-1 from Venezuela, the extensive genetic
       heterogeneity observed reinforces the interest in characterizing
       additional HIV-1 isolates worldwide for adequate vaccine design.
 DE    Adult  Amino Acid Sequence  Base Sequence  DNA Primers  Genes, env
       Genes, pol  Human  HIV Envelope Protein gp120/*GENETICS  HIV
       Infections/BLOOD/*VIROLOGY  HIV-1/CLASSIFICATION/*GENETICS/ISOLATION &
       PURIF  Male  Middle Age  Molecular Sequence Data  Mutation  Phylogeny
       RNA-Directed DNA Polymerase/*GENETICS  Sequence Homology, Amino Acid
       Support, Non-U.S. Gov't  Venezuela  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

