       Document 1031
 DOCN  M9621031
 TI    Intrapatient variability of the human immunodeficiency virus type 2
       envelope V3 loop.
 DT    9602
 AU    Sankale JL; de la Tour RS; Renjifo B; Siby T; Mboup S; Marlink RG; Essex
       ME; Kanki PJ; Department of Cancer Biology, Harvard School of Public
       Health,; Boston, Massachusetts 02115, USA.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):617-23. Unique Identifier :
       AIDSLINE GENBANK/U24388
 AB    Studies of HIV-2 infection have shown lower rates of sexual and
       perinatal transmission and a prolonged incubation period to AIDS as
       compared to HIV-1. To evaluate the role of genetic variation in HIV
       pathogenesis, we studied intrapatient variability in the V3 loop of the
       HIV-2 envelope gene over time in five seropositive individuals. Proviral
       sequences derived from uncultured PBMC DNA (n = 102) demonstrated an
       average sequence heterogeneity within a sample of 1.4% (0-4.1%). This
       was significantly lower than the V3 sequence heterogeneity observed in
       HIV-1, which can be as high as 6.1%. In HIV-2-seropositive healthy
       patients the average intrapatient nucleotide variability rate was 0.6%
       compared to 2.0% in patients with clinical AIDS. The lower rate of
       variability between HIV-2 and HIV-1 is compatible with differences in
       transmission and pathogenesis of these two related viruses.
 DE    Amino Acid Sequence  Base Sequence  CD4 Lymphocyte Count  DNA Primers
       Female  Follow-Up Studies  Human  HIV Envelope Protein gp120/*GENETICS
       HIV Infections/BLOOD/PHYSIOPATHOLOGY/*VIROLOGY  HIV-1/GENETICS
       HIV-2/*GENETICS/ISOLATION & PURIF  Male  Molecular Sequence Data
       Peptide Fragments/*GENETICS  Polymerase Chain Reaction  Sequence
       Homology, Amino Acid  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  Support, U.S. Gov't, P.H.S.  *Variation (Genetics)  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

