       Document 1030
 DOCN  M9621030
 TI    Nucleotide sequence and restriction fragment length polymorphism
       analysis of the long terminal repeat of human T cell leukemia virus type
       II.
 DT    9602
 AU    Eiraku N; Monken C; Kubo T; Zhu SW; Rios M; Bianco C; Hjelle B;
       Nagashima K; Hall WW; Department of Medical Virology, Rockefeller
       University, New York,; New York 10021, USA.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):625-36. Unique Identifier :
       AIDSLINE GENBANK/L37146
 AB    Molecular studies have demonstrated the existence of two major subtypes
       of human T cell leukemia virus type II: HTLV-IIa and HTLV-IIb. In
       attempts to further classify this family of viruses we have carried out
       nucleotide sequence and restriction fragment length polymorphism (RFLP)
       analysis of the long terminal repeat (LTR), a region that has been shown
       in previous studies to have the greatest intra- and intersubtype genomic
       divergence. Analysis of the nucleotide sequences suggested the existence
       of distinct phylogenetic groups in each subtype and, on the basis of
       predicted differences in restriction endonuclease sites, RFLP analysis
       allowed the identification of four groups within the IIa subtype (a1-a4)
       and six within the IIb subtype (b1-b6). Nucleotide sequence analysis
       also suggested the possible existence of HTLV-II quasispecies. However,
       this appeared not to be significant, and preliminary studies suggest
       that these would not be expected to influence the results of RFLP
       analysis appreciably. The validity of the RFLP method was demonstrated
       in an analysis of 36 randomly chosen samples from HTLV-II seropositive
       blood donors from the New York City Blood Center, where it could be
       shown that all could be successfully classified. Moreover, the RFLP
       analysis correctly matched the viruses in donors and recipients of
       contaminated blood in four situations in which HTLV-II was inadvertently
       transmitted by transfusion. RFLP analysis of the LTR appears to be a
       rapid and reliable method by which to identify HTLV-II infection. This
       should prove useful in studies of the epidemiology and the
       characterization of viruses present both in nonindigenous and indigenous
       populations.
 DE    Base Sequence  DNA, Viral/GENETICS  Human  HTLV-BLV
       Infections/BLOOD/VIROLOGY  HTLV-II/CLASSIFICATION/*GENETICS/ISOLATION &
       PURIF  Indians, North American  Molecular Sequence Data  Phylogeny
       *Polymorphism, Restriction Fragment Length  Repetitive Sequences,
       Nucleic Acid/*GENETICS  RNA, Viral/GENETICS  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

