       Document 1024
 DOCN  M9621024
 TI    Molecular cloning and characterization of a TAR-binding nuclear factor
       from T cells.
 DT    9602
 AU    Reddy TR; Suhasini M; Rappaport J; Looney DJ; Kraus G; Wong-Staal F;
       Department of Medicine, University of California, San Diego, La; Jolla
       92093-0665, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Jun;11(6):663-9. Unique Identifier :
       AIDSLINE GENBANK/L22453
 AB    The TAt protein of the human immunodeficiency virus type 1 (HIV-1)
       activates the expression of viral mRNA through a cis-acting element in
       the LTR termed TAR. TAR RNA forms a stable stem-loop structure.
       Mutagenesis studies indicate that the stem structure, the primary
       sequence of the loop, and three unpaired bases in the stem (bulge) are
       important for Tat activation. Using the in vitro-transcribed TAR RNA as
       a probe, we have cloned a gene (TARBP-b) that encodes a TAR-binding
       protein from a cDNA expression library derived from Hut-78 cells.
       Expression of the 1.4-kb TARBP-b mRNA was observed in all mammalian cell
       lines tested. TARBP-b binds specifically to the bulge region of TAR RNA
       and trans-activates the HIV-1 long terminal repeat in the presence of
       ptat and prev expression plasmids. These results suggest that TARBP-b
       contributes to tat-mediated trans-activation.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Gene Expression
       Regulation, Viral/GENETICS  Gene Products, rev/PHYSIOLOGY  Gene
       Products, tat/PHYSIOLOGY  Genes, Viral/GENETICS  Human  HIV Long
       Terminal Repeat/GENETICS  HIV-1/*GENETICS  Lymphocytes/VIROLOGY  Mice
       Molecular Sequence Data  Nucleic Acid Conformation  RNA Probes
       RNA-Binding Proteins/CHEMISTRY/*GENETICS  RNA, Messenger/BIOSYNTHESIS
       RNA, Viral/CHEMISTRY/*METABOLISM  Sequence Analysis, DNA  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes/*VIROLOGY  Trans-Activation
       (Genetics)/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

