       Document 1017
 DOCN  M9621017
 TI    Local synthesis of IgG antibodies to HIV within the female and male
       genital tracts during asymptomatic and pre-AIDS stages of HIV infection.
 DT    9602
 AU    Belec L; Tevi-Benissan C; Lu XS; Prazuck T; Pillot J; Unite
       d'Immunologie Microbienne, Institut Pasteur, Paris,; France.
 SO    AIDS Res Hum Retroviruses. 1995 Jun;11(6):719-29. Unique Identifier :
       AIDSLINE MED/96078233
 AB    Paired sera and cervicovaginal secretions or seminal fluids, obtained
       from HIV-1-infected, clinically asymptomatic women (n = 41) and men (n =
       12), were investigated in order to test the hypothesis of a local
       synthesis of IgG to HIV in the female and male reproductive tracts.
       Anti-gp41 + p24 IgG was evaluated by an IgG immunocapture assay, and
       anti-gp160 IgG by an indirect ELISA. Estimation of anti-HIV IgG-specific
       activities was carried out after ponderal determination of total IgG and
       evaluation of anti-HIV IgG activity. IgG to gp41 + p24, as well as IgG
       to gp160, were specifically detected in all sera, cervicovaginal
       secretions, and seminal fluid samples from all tested HIV-1-infected
       subjects. The mean specific activities of IgG to gp41 + p24 in
       cervicovaginal secretions and in seminal fluids were about 33-fold (in
       women) and 16-fold (in men) that of the corresponding sera; similarly,
       the mean specific activities of IgG to gp160 in genital secretions were
       about 17-fold (in women) and 10-fold (in men) that of the corresponding
       sera. IgGs to HIV are constantly detected in genital secretions from
       HIV-1-infected subjects, and appear to be largely synthesized in situ
       within the genital tract of both genders.
 DE    Adolescence  Adult  Antibody Affinity  Cervix Uteri/IMMUNOLOGY/SECRETION
       Enzyme-Linked Immunosorbent Assay/METHODS  Female  Gene Products,
       env/IMMUNOLOGY  Genitalia/*IMMUNOLOGY  Human  HIV
       Antibodies/ANALYSIS/*BIOSYNTHESIS/BLOOD  HIV Core Protein p24/IMMUNOLOGY
       HIV Envelope Protein gp41/IMMUNOLOGY  HIV Infections/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  IgG/ANALYSIS/*BIOSYNTHESIS/BLOOD  Male  Matched-Pair
       Analysis  Middle Age  Protein Precursors/IMMUNOLOGY  Semen/IMMUNOLOGY
       Support, Non-U.S. Gov't  Vagina/IMMUNOLOGY/SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

