       Document 0943
 DOCN  M9620943
 TI    Effects of T-helper 2-type cytokines, interleukin-3 (IL-3), IL-4, IL-5,
       and IL-6 on the survival of cultured human mast cells.
 DT    9602
 AU    Yanagida M; Fukamachi H; Ohgami K; Kuwaki T; Ishii H; Uzumaki H; Amano
       K; Tokiwa T; Mitsui H; Saito H; et al; Pharmaceutical Development
       Laboratory, Kirin Brewery Co, Ltd,; Gunma, Japan.
 SO    Blood. 1995 Nov 15;86(10):3705-14. Unique Identifier : AIDSLINE
       MED/96068735
 AB    Although stem cell factor (SCF) has been identified as a critical
       cytokine for the development of human mast cells from their progenitors,
       the effects of other cytokines on human mast cells are less well
       understood. We examined the effects of several cytokines on the survival
       of human mast cells of 100% purity generated in suspension cultures of
       umbilical cord blood mononuclear cells in the presence of 100 ng/mL
       recombinant human (rh) SCF and interleukin-6 (IL-6). Mast cells
       suspended in conventional serum-containing medium died over a period of
       2 to 6 days after the withdrawal of SCF and IL-6. The cells became
       pyknotic and underwent DNA fragmentation characteristic of apoptosis.
       The addition of SCF, IL-3, IL-4, IL-5, or IL-6 to the cultures in both
       serum-containing and serum-free medium prolonged their survival in a
       dose-dependent manner. Some other cytokines, such as IL-2, IL-9, IL-10,
       IL-11, tumor necrosis factor-alpha, transforming growth factor-beta 1,
       and nerve growth factor, had no survival-promoting effect at 100 ng/mL.
       Preincubation of mast cells with SCF, IL-4, IL-5, or IL-6 for 24 hours
       during sensitization with IgE enhanced IgE/anti-IgE antibody-induced
       histamine release from mast cells, whereas IL-3 showed a negligible
       effect. Polymerase chain reaction amplification of alpha-chains of IL-3
       receptor (R), IL-4 R, IL-5 R, and IL-6 R yielded products of the correct
       size predicted from the sequence of each receptor. The binding assay
       using 125I-labeled IL-3 indicated that these mast cells bear receptors
       for IL-3. These findings suggest that IL-3, Il-4, IL-5, and IL-6, which
       are mainly produced by T-helper 2 lymphocytes, might regulate the
       functions of human mast cells in vivo via specific receptors in allergic
       reactions.
 DE    Antibodies, Anti-Idiotypic/PHARMACOLOGY  Antibodies,
       Monoclonal/IMMUNOLOGY/PHARMACOLOGY  Antigens, CD/BIOSYNTHESIS/GENETICS
       Apoptosis/*DRUG EFFECTS  Base Sequence  Cell Survival  Cells, Cultured
       Comparative Study  Fetal Blood/CYTOLOGY  Gene Expression  Histamine
       Release/DRUG EFFECTS  Human  IgE/IMMUNOLOGY  Interleukin-3/PHARMACOLOGY
       Interleukin-4/PHARMACOLOGY  Interleukin-5/PHARMACOLOGY
       Interleukin-6/PHARMACOLOGY  Interleukins/*PHARMACOLOGY/SECRETION  Mast
       Cells/CYTOLOGY/*DRUG EFFECTS  Molecular Sequence Data  Polymerase Chain
       Reaction  Receptors, Interleukin/BIOSYNTHESIS/GENETICS  Receptors,
       Interleukin-3/BIOSYNTHESIS/GENETICS  Recombinant Proteins/PHARMACOLOGY
       RNA, Messenger/BIOSYNTHESIS/GENETICS  Stem Cell Factor/*PHARMACOLOGY
       Th2 Cells/*SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

