       Document 0902
 DOCN  M9620902
 TI    Which nutrient supplements for optimal HIV-1 production by cultured
       human lymphocytes?
 DT    9602
 AU    Landureau JC; Achour A; Fouchard M; M'Bika JP; Maziere JC; Zagury D;
       Laboratoire de Physiologie Cellulaire, Universite Pierre et; Marie
       Curie, Paris, France.
 SO    Cell Mol Biol (Noisy-le-grand). 1995 May;41(3):423-30. Unique Identifier
       : AIDSLINE MED/96059404
 AB    Under standard conditions, all established cell-lines of HIV-1-infected
       lymphocytes are cultured in a medium containing a high percentage of
       serum. We have found that whilst partial or total serum depletion
       impedes their cell growth, it dramatically increases their virus
       production. Serial subcultures in serum-depleted medium are necessary to
       insure permanent cell growth and a relatively high reverse transcriptase
       activity. Following optimization of culture conditions, both
       HIV-infected and uninfected H9 cells have been regularly grown in a new
       protein-free medium. Such chemically defined culture conditions were
       required to detect the effect of minor components, such as vitamins, on
       host cell-virus interactions.
 DE    Blood Proteins  Cell Division  Cell Line  Cholesterol/PHARMACOLOGY
       *Culture Media  Culture Media, Serum-Free  DNA, Viral/BIOSYNTHESIS
       Human  HIV-1/*GROWTH & DEVELOPMENT  Multivariate Analysis
       Riboflavin/PHARMACOLOGY  RNA-Directed DNA Polymerase/METABOLISM  Time
       Factors  Virus Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

