       Document 0878
 DOCN  M9620878
 TI    Release of hydrogen peroxide from human T cell lines and normal
       lymphocytes co-infected with HIV-1 and mycoplasma.
 DT    9602
 AU    Chochola J; Strosberg AD; Stanislawski M; Departement des Maladies
       Infectieuses, Parasitaires et; Tropicales, INSERM U313, CHU
       Pitie-Salpetriere, Paris, France.
 SO    Free Radic Res. 1995 Sep;23(3):197-212. Unique Identifier : AIDSLINE
       MED/96002901
 AB    Human T-cell lines and normal lymphocytes persistently or acutely
       co-infected with the human immunodeficiency virus type 1 (HIV-1) and
       mycoplasmas were found to release hydrogen peroxide (H2O2), a likely
       cause of oxidative stress in these cells. The spectrofluorometric
       measurement of H2O2 release from these cells, using the scopoletin
       fluorescence quenching technique, gave values of 16-84 p moles/10(6)
       cells/min. In CEM cells, H2O2 was released only when acutely co-infected
       with HIV-1 and mycoplasmas, and not when infected with either organism
       alone. Anti-mycoplasmal antibiotics strongly reduced H2O2 release, and
       improved cell viability without blocking virus replication. These
       results suggest that the simultaneous infection by HIV-1 and mycoplasma
       leads to the release of H2O2, a toxic and potentially lethal metabolite,
       which in vivo may contribute to HIV-1 pathogenicity.
 DE    Cell Line  Fluorescence  Human  Hydrogen Peroxide/*METABOLISM  HIV
       Infections/*METABOLISM  *HIV-1  *Mycoplasma  Mycoplasma
       Infections/*METABOLISM  Oxidative Stress  Support, Non-U.S. Gov't
       T-Lymphocytes/*METABOLISM/MICROBIOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

