       Document 0877
 DOCN  M9620877
 TI    Gel electrophoretic analysis of cellular and secreted proteins from
       resting and activated rat alveolar macrophages treated with pentamidine
       isethionate.
 DT    9602
 AU    Selkirk JK; He C; Merrick BA; Laboratory of Molecular Carcinogenesis,
       National Institute of; Environmental Health Sciences, Research Triangle
       Park, NC 27709,; USA.
 SO    J Chromatogr A. 1995 Sep 22;711(2):331-7. Unique Identifier : AIDSLINE
       MED/96014393
 AB    Pneumocystic carinii pneumonia, which is a major cause of death among
       patients suffering from acquired immunodeficiency syndrome, has often
       been treated successfully with pentamidine isethionate. This study
       examines pentamidine effects on cellular and secreted proteins from rat
       alveolar macrophages by two-dimensional gel electrophoresis and
       computerized image analysis. Over 100 secreted proteins were detected by
       fluorography. Fluorography showed pentamidine diminished tumor necrosis
       factor and interleukin-1 release along with other proteins. Effects of
       combined bacterial lipopolysaccharide and pentamidine were more
       pronounced on secreted versus cellular proteins in protein amount and
       pattern difference. Thus pentamidine exhibited a general repressive
       effect on cellular and secreted protein expression in resting and
       activated macrophages.
 DE    Animal  Antifungal Agents/*PHARMACOLOGY  Cells, Cultured
       Electrophoresis, Gel, Two-Dimensional/*METHODS  Female  Macrophages,
       Alveolar/CHEMISTRY/*DRUG EFFECTS/SECRETION  Pentamidine/*PHARMACOLOGY
       Proteins/*ANALYSIS/SECRETION  Rats  Rats, Inbred F344  Spectrometry,
       Fluorescence  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

