       Document 0874
 DOCN  M9620874
 TI    Ribosomal internal transcribed spacer sequences are identical among
       Mycobacterium avium-intracellulare complex isolates from AIDS patients,
       but vary among isolates from elderly pulmonary disease patients.
 DT    9602
 AU    De Smet KA; Brown IN; Yates M; Ivanyi J; MRC Tuberculosis and Related
       Infections Unit, Hammersmith; Hospital, London, UK.
 SO    Microbiology. 1995 Oct;141 ( Pt 10):2739-47. Unique Identifier :
       AIDSLINE GENBANK/X74056
 AB    Sequencing 280 bp of the internal transcribed spacer (ITS) between the
       16S and 23S rRNA genes in a collection of 46 clinical isolates of the
       Mycobacterium avium-intracellulare complex (MAI complex) identified nine
       different sequences, grouping these isolates in nine 'ITS sequevars'.
       This analysis extends the subdivision within the MAI complex to 18 ITS
       sequevars and also improves discrimination from other mycobacterial
       species. Evaluation of the sequevar grouping among different clinical
       sources revealed strong association of the M. avium sequevar Mav-B with
       AIDS and with lymphadenitis in children (18 out of 20 and 3 out of 3
       respectively). Isolates from elderly patients with pulmonary disease and
       not suspected of being HIV infected belonged predominantly to M.
       intracellulare ITS sequevars and sequevars not assigned to either M.
       avium or M. intracellulare. On the other hand, animal isolates were of
       both the Mav-A and Mav-B sequevars. We conclude that ITS sequevar typing
       is an accurate way of identifying distinct MAI complex strains. The
       observed differences between clinical sources suggest that ITS sequevars
       reflect possibly important, biologically and clinically relevant
       polymorphisms between MAI complex organisms.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY  Aged
       Animal  Base Sequence  Child  Comparative Study  DNA, Bacterial/GENETICS
       DNA, Ribosomal/*GENETICS  Genes, Bacterial  Human  Lung
       Diseases/COMPLICATIONS/*MICROBIOLOGY  Molecular Sequence Data
       Mycobacterium avium/GENETICS  Mycobacterium avium
       Complex/CLASSIFICATION/*GENETICS/IMMUNOLOGY  Mycobacterium
       avium-intracellulare Infection/COMPLICATIONS/  *MICROBIOLOGY  Phylogeny
       RNA, Ribosomal, 16S/GENETICS  Sequence Analysis, DNA  Sequence Homology,
       Nucleic Acid  Serotyping  Tuberculosis/VETERINARY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

