       Document 0852
 DOCN  M9620852
 TI    Effect of antibody to HIV-1 Tat protein on viral replication in vitro
       and progression of HIV-1 disease in vivo.
 DT    9602
 AU    Re MC; Furlini G; Vignoli M; Ramazzotti E; Roderigo G; De Rosa V; Zauli
       G; Lolli S; Capitani S; La Placa M; Institute of Microbiology,
       University of Bologna Medical School,; St. Orsola General Hospital,
       Italy.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Dec 1;10(4):408-16.
       Unique Identifier : AIDSLINE MED/96074273
 AB    In HIV-1-infected cell cultures, a relatively low concentration (5
       micrograms/ml) of monoclonal antibody (mAb) against
       HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1
       replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral
       blood mononuclear cell (PBMC) cultures and significantly reduced the
       expression of a Tat-responsive CAT-reporter construct in
       HIV-1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a
       significant reduction and a consistent delay in HIV-1 replication when
       added to PBMCs from HIV-1-infected patients cocultivated with
       phytohemagglutinin (PHA)-stimulated normal PBMCs. These data indicate
       that an autocrine-paracrine loop sustained by extracellular Tat protein,
       which is actively released by HIV-1-infected cells, may affect HIV-1
       replication in cell cultures in vitro. An inverse relationship between
       natural anti-Tat antibody levels and p24 antigenemia was demonstrated by
       retrospective analysis of serial serum samples obtained from 10
       HIV-1-seropositive hemophiliac patients followed over a 7-9-year period.
       This datum points to a possible influence of anti-Tat antibody on the
       progression of HIV-1 disease in vivo. These findings have strong
       implications for Tat protein as a possible target for specific
       immunotherapy in HIV-1-infected patients.
 DE    Antibodies, Monoclonal/IMMUNOLOGY/*PHARMACOLOGY  Cell Line  Cells,
       Cultured  Chloramphenicol Acetyltransferase/BIOSYNTHESIS  Disease
       Progression  Gene Products, tat/*IMMUNOLOGY  Hemophilia/COMPLICATIONS
       Human  HIV Antibodies/IMMUNOLOGY/*PHARMACOLOGY  HIV Core Protein
       p24/IMMUNOLOGY  HIV Infections/COMPLICATIONS/*IMMUNOLOGY/PHYSIOPATHOLOGY
       HIV-1/IMMUNOLOGY/*PHYSIOLOGY  Leukocytes, Mononuclear/VIROLOGY
       Repetitive Sequences, Nucleic Acid  Support, Non-U.S. Gov't
       Trans-Activation (Genetics)  Transfection  *Virus Replication/DRUG
       EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

