       Document 0833
 DOCN  M9620833
 TI    Decreased T-cell proliferative response to common environmental antigens
       could be an indicator of early human immunodeficiency virus-mediated
       lymphocyte lesions.
 DT    9602
 AU    Tassinari P; Deibis L; Blanca I; Bianco NE; Echeverria de Perez G;
       Instituto de Inmunologia, Facultad de Medicina, Universidad; Central de
       Venezuela, Caracas.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):404-7. Unique Identifier :
       AIDSLINE MED/96082414
 AB    To evaluate CD4+/CD29+ cells and their responses to different antigens
       in polar stages of human immunodeficiency virus (HIV) infection, we
       studied 26 HIV-seropositive carriers (SPCs) and 15 patients with AIDS
       simultaneously with 20 healthy volunteers (HVs) and 10 seronegative
       homosexual and bisexual men (SNH). CD3, CD4, CD29, and CD45RA phenotypes
       were analyzed by two-color flow cytometry. Significant depletion of CD4+
       T cells and both memory (CD4+/CD29+) and naive (CD4+/CD45RA+) T-cell
       subsets was found among SPCs and AIDS patients compared with the numbers
       of such cells in the HV and SNH groups. Responses to optimal doses of
       Candida albicans, streptokinase, and tetanus toxoid were explored in
       peripheral blood mononuclear cells and CD4(+)- and CD4+/CD29(+)-enriched
       cell populations. In SPCs, the response to C. albicans in peripheral
       blood mononuclear cells showed a statistically significant diminution
       compared with the response of HVs (15,308 versus 35,951 cpm). In
       addition, a significantly reduced response to streptokinase was evident
       only when cell preparations were CD4+/CD29+ enriched (3,048 versus
       10,367 cpm). Furthermore, the SPC group comprised seven responders to at
       least one antigen and seven nonresponders to any of the selected
       specific antigens. Absence of a response in these latter patients was
       independent of the absolute counts of memory and naive T-cell
       populations. The response to tetanus toxoid, although diminished in
       SPCs, was not significantly different from that in controls. Our results
       suggest that defective responses to common environmental antigens,
       unrelated to the absolute number of CD4+/CD29+ cells, is probably an
       early indicator of an HIV-induced lymphocyte lesion.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY/PATHOLOGY  Adult
       Antigens, CD3  Antigens, CD45  Antigens, Fungal/IMMUNOLOGY  Candida
       albicans/IMMUNOLOGY  Human  HIV Seropositivity/*IMMUNOLOGY/PATHOLOGY
       HIV-1/*IMMUNOLOGY  Immunologic Memory/IMMUNOLOGY  *Lymphocyte
       Transformation  Male  Middle Age  Prognosis  Streptokinase/IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY/PATHOLOGY
       Tetanus Toxoid/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

