       Document 0829
 DOCN  M9620829
 TI    Anti-human immunodeficiency virus type 1 antibodies of noninfected
       subjects are not related to autoantibodies occurring in systemic
       diseases.
 DT    9602
 AU    Kammerer R; Burgisser P; Frei PC; Division of Immunology and Allergy,
       Centre Hospitalier; Universitaire Vaudois, Lausanne, Switzerland.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):458-61. Unique Identifier :
       AIDSLINE MED/96082424
 AB    Indeterminate Western blot (WB) (immunoblot) patterns for anti-human
       immunodeficiency virus type 1 (HIV-1) antibodies are often observed when
       testing serum samples from noninfected individuals. We investigated here
       the possible involvement of some frequently occurring autoantibodies
       (anti-SmB/B', U1snRNP [68 kDa, A, and C], Ro/SS-A [60 and 52 kDa], and
       Jo-1) in the generation of such indeterminate HIV-1 WB. In particular,
       the role of a reported sequence homology between p24 gag and the SmB/B'
       autoantigen was investigated. Serum samples were obtained from 50
       healthy controls, 51 patients with systemic lupus erythematosus (SLE),
       46 with systemic sclerosis, 6 with Sjogren's disease, 3 with mixed
       connective tissue disease, and 41 healthy subjects with persistent
       indeterminate HIV-1 WB. Reactivity to HIV-1 p24 gag was slightly but not
       significantly more frequent in patients with SLE than in controls (25.5%
       versus 14.0%; P > 0.1), whereas reactivity to HIV-1 p17 gag was
       significantly more frequent in the former subjects (23.5% versus 8.0%; P
       = 0.03). Simultaneous reactivity to p17 and p24 was observed in patients
       with SLE (11.8%; P = 0.014) or systemic sclerosis (8.7%; P = 0.049) but
       not in controls. There was no association found between the presence of
       any autoantibody and the occurrence of indeterminate HIV-1 WB nor
       between the presence of p24-reactive antibodies and anti-SmB/B'; this
       indicates that most p24-reactive antibodies are directed to epitopes
       other than the proline-rich sequences shared by p24 gag and SmB/B'.
 DE    Aged  Autoantibodies/*BLOOD  Autoantigens/IMMUNOLOGY  Autoimmune
       Diseases/*IMMUNOLOGY  Blotting, Western  Cross Reactions  Human  HIV
       Antibodies/BLOOD/*IMMUNOLOGY  HIV Core Protein p24/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS  HIV-1/*IMMUNOLOGY  Male  Predictive Value of
       Tests  Ribonucleoproteins, Small Nuclear/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

