       Document 0825
 DOCN  M9620825
 TI    Multi-gene family of major surface glycoproteins of Pneumocystis
       carinii: full-size cDNA cloning and expression.
 DT    9602
 AU    Kitada K; Wada M; Nakamura Y; Department of Tumor Biology, University of
       Tokyo, Japan.
 SO    DNA Res. 1994;1(2):57-66. Unique Identifier : AIDSLINE GENBANK/D17440
 AB    The major surface glycoprotein (MSG) of Pneumocystis carinii plays a
       crucial role in the fatal pneumonia caused by this organism in AIDS
       patients. A cDNA encoding a full-length MSG polypeptide was isolated
       from a lambda library of rat-derived P. carinii cDNAs. The deduced MSG,
       referred to as the MSG5 subtype, is a 120,765-Da protein composed of
       1,076 amino acids and contains an anchoring hydrophobic sequence at the
       C-terminus of the protein. Sequence analyses of cloned MSG-cDNAs
       revealed an MSG-gene family with approximately 70% protein sequence
       identity between subtypes. P. carinii karyotype hybridization analyses
       indicated that the MSG gene family members are scattered throughout most
       of the P. carinii chromosomes. These recombinant MSG proteins reacted
       with the antiserum from P. carinii-infected rats, as expected, and
       antiserum generated against P. carinii-infected mice, indicating the
       existence of common determinants in MSG polypeptides. The family of MSG
       proteins is rich in cysteine residues and these cysteines are highly
       conserved in all MSG subtypes regardless of species specificity,
       suggesting the structural and/or functional importance of these
       cysteines. The pathobiological significance of the MSG gene family and
       its sequence diversity in P. carinii is discussed.
 DE    Amino Acid Sequence  Animal  Antibodies, Fungal/IMMUNOLOGY  Antigens,
       Fungal/*GENETICS  Base Sequence  Cloning, Molecular  Comparative Study
       DNA Primers/CHEMISTRY  DNA, Complementary/GENETICS  Ferrets/MICROBIOLOGY
       Fungal Proteins/*GENETICS  Gene Expression Regulation, Fungal  Genes,
       Reiterated  Membrane Glycoproteins/*GENETICS  Mice  Molecular Sequence
       Data  Pneumocystis carinii/*GENETICS  Rats  Recombinant
       Proteins/IMMUNOLOGY  Sequence Alignment  Sequence Homology, Amino Acid
       Sequence Homology, Nucleic Acid  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

