       Document 0805
 DOCN  M9620805
 TI    Cytokine expression in large cell lymphoma associated with acquired
       immunodeficiency syndrome.
 DT    9602
 AU    Marsh JW; Herndier B; Tsuzuki A; Ng VL; Shiramizu B; Abbey N; McGrath
       MS; Department of Medicine, University of California, San Francisco;
       94110, USA.
 SO    J Interferon Cytokine Res. 1995 Mar;15(3):261-8. Unique Identifier :
       AIDSLINE MED/96075715
 AB    Cytokine expression was examined by reverse transcriptase-polymerase
       chain reaction (RT-PCR) in a retrospective sampling of 16
       AIDS-associated large cell lymphomas (LCL). IL-6 receptor (IL-6R) and
       IL-10 expression was detected in a majority of the tumor specimens
       tested, IL-6 expression was detected in 5 of 16 lymphomas that also
       expressed IL-6R, suggestive of an autocrine mechanism of disease. A
       subset of tumor samples described as mixed immunophenotype contained
       large numbers of infiltrating T lymphocytes and macrophages.
       Immunoperoxidase staining of a representative tumor of mixed
       immunophenotype demonstrated the presence of HIV-infected macrophages
       that also stained with anti-IL-6. This finding suggests that IL-6
       produced by nonlymphoid cells may act as a paracrine growth factor for
       tumor cells that express IL-6R. Although earlier studies of AIDs
       burkitt's lymphoma cell lines suggested that IL-10 expression required
       EBV infection, 7 of 12 AIDS LCLs that expressed IL-10 did so in the
       absence of EBV by EBER in situ hybridization. Because AIDS LCLs
       frequently express cell surface CD5, we speculate that IL-10 may act as
       an autocrine or paracrine growth factor for this class of lymphoma.
       These studies suggest that IL-6 and IL-10 are involved in the
       pathogenesis of AIDS-associated large cell and mixed immunophenotype
       lymphoma.
 DE    Base Sequence  Cytokines/*BIOSYNTHESIS/GENETICS  Herpesvirus 4,
       Human/GENETICS/*ISOLATION & PURIF  Human  Immunoenzyme Techniques
       Interleukin-10/BIOSYNTHESIS/GENETICS
       Interleukin-6/BIOSYNTHESIS/GENETICS  Lymphocytes,
       Tumor-Infiltrating/IMMUNOLOGY  Lymphoma,
       AIDS-Related/COMPLICATIONS/*IMMUNOLOGY/VIROLOGY  Lymphoma,
       Large-Cell/COMPLICATIONS/*IMMUNOLOGY/VIROLOGY  Molecular Sequence Data
       Retrospective Studies  RNA/BIOSYNTHESIS  RNA, Viral/ANALYSIS  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

