       Document 0724
 DOCN  M9620724
 TI    Investigation of the complement receptor 3 (CD11b/CD18) in human rectal
       epithelium.
 DT    9602
 AU    Hussain LA; Kelly CG; Rodin A; Jourdan M; Lehner T; Department of
       Immunology, United Medical School, Guy's Hospital,; London, UK.
 SO    Clin Exp Immunol. 1995 Nov;102(2):384-8. Unique Identifier : AIDSLINE
       MED/96069892
 AB    Rectal and cervicovaginal mucosa are common routes of transmission of
       HIV, although the mechanism of transmission is unknown. We have
       investigated human rectal and cervicovaginal epithelia for the
       expression of complement receptors (CR) and cell adhesion molecules
       which may be involved in HIV and other infections. In rectal mucosa, CR3
       was detected in the surface and crypt epithelial cells by
       immunohistology, using MoAbs to CD18 and CD11b in 10 out of 15
       specimens. RNA transcripts encoding both CD11b and CD18 were also
       demonstrated in surface and crypt epithelial cells by in situ
       hybridization. Although CD11b was detected in the epithelial cells in
       three out of the 14 cervicovaginal specimens, we were unable to detect
       CD18. We suggest that expression of the CD11b/CD18 heterodimer might
       facilitate transmission of HIV by enhancing binding of HIV-antibody
       complexes in seminal fluid to epithelial cells. Alternatively, since
       intercellular adhesion molecule-1 (ICAM-1) is a receptor for CD11b/CD18,
       this may promote adhesion between epithelial cells and HIV-infected
       mononuclear cells in seminal fluid.
 DE    Antigens, CD18/*METABOLISM  Cell Adhesion Molecules/METABOLISM  Cervix
       Uteri/IMMUNOLOGY  Epithelium/IMMUNOLOGY  Female  Gene Expression  Human
       Immunoenzyme Techniques  In Situ Hybridization  Intercellular Adhesion
       Molecule-1/METABOLISM  Intestinal Mucosa/METABOLISM  Macrophage-1
       Antigen/*METABOLISM  Rectum/*IMMUNOLOGY  RNA, Messenger/GENETICS
       Support, Non-U.S. Gov't  Vagina/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

