       Document 0679
 DOCN  M9620679
 TI    Assessment of aerosol containment on the ELITE flow cytometer.
 DT    9602
 AU    Ferbas J; Chadwick KR; Logar A; Patterson AE; Gilpin RW; Margolick JB;
       Department of Infectious Diseases and Microbiology, Graduate; School of
       Public Health, University of Pittsburgh, Pennsylvania,; USA.
 SO    Cytometry. 1995 Mar 15;22(1):45-7. Unique Identifier : AIDSLINE
       MED/96090282
 AB    Biohazardous aerosols generated during cell sorting have been of
       increased concern recently because of interest in sorting specimens
       containing human immunodeficiency virus type 1 (HIV-1). Current flow
       cytometers have features designed to contain such aerosols within the
       sorting chamber, but the efficacy of these features has not been
       established. Therefore, we tested aerosol containment by two ELITE flow
       cytometers (Coulter Cytometry, Inc., Hialeah, FL) during sorting of
       specimens containing high titers of bacteriophage. Agar plates confluent
       with susceptible Escherichia coli were used to detect infectious units
       released from the sorting chamber. Under recommended operating
       conditions very few infectious units were released from the sorting
       chambers. Release increased when the center stream was not optimally
       collected in a vacuum-exhausted tube or the chamber door was not
       completely closed. Failure of the negative pressure and high efficiency
       particle air (HEPA) filtration features had less of an effect. The data
       indicate that these standard safety features provide a rational
       expectation of safety for the flow cytometry operator.
 DE    Aerosols  Cell Separation/INSTRUMENTATION  *Containment of Biohazards
       Evaluation Studies  Flow Cytometry/*INSTRUMENTATION  Human
       HIV-1/ISOLATION & PURIF  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

