       Document 0678
 DOCN  M9620678
 TI    CD4 Lymphocyte enumeration: comparison between flow cytometry and enzyme
       immunoassay.
 DT    9602
 AU    Cordiali Fei P; Solmone M; Vanacore P; Giglio A; Bonifati C; Carducci M;
       Mussi A; Chiarotti F; Ameglio F; Laboratory of Clinical Pathology,
       Istituto San Gallicano, Rome,; Italy.
 SO    Cytometry. 1995 Mar 15;22(1):70-4. Unique Identifier : AIDSLINE
       MED/96090286
 AB    Assessment of the CD4 lymphocyte number, currently performed by flow
       cytometry, is one of the main laboratory tests for establishing
       progression to acquired immunodeficiency syndrome (AIDS). An enzyme
       immunoassay has recently been commercialized which can be very useful
       for counting CD4 cells in laboratories where flow cytometers are not
       available. In the present study, a comparative evaluation of CD4
       positive lymphocytes with flow cytometry and the enzyme assay was made
       in healthy subjects (N = 30, R = 0.88, P < 0.0001), human
       immunodeficiency virus (HIV)-infected individuals (N = 80, R = 0.91, P <
       0.0001), and patients with autoimmune diseases (N = 28, R = 0.82, P <
       0.001) or psoriasis (N = 18, R = 0.76, P = 0.01). A correlation between
       the two methodologies was not found in psoriatic patients under
       treatment with cyclosporin A (N = 7, R = 0.05, not significant). Some
       differences could be found at low CD4 lymphocyte levels since the
       influence of CD4 antigen eluted from monocytes or soluble CD4 in the
       whole blood sample could cause overestimation of CD4 cell numbers by the
       enzyme assay.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS  Autoimmune
       Diseases/*DIAGNOSIS  Case-Control Studies  Comparative Study  CD4
       Lymphocyte Count/*METHODS  Disease Progression  *Flow Cytometry  Human
       *Immunoenzyme Techniques  Linear Models  Psoriasis/*DIAGNOSIS  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

