       Document 0655
 DOCN  M9620655
 TI    Value of specific immunoglobulin A detection by two immunocapture assays
       in the diagnosis of toxoplasmosis.
 DT    9602
 AU    Foudrinier F; Marx-Chemla C; Aubert D; Bonhomme A; Pinon JM; Laboratoire
       de Parasitologie-Mycologie, Institut National de la; Sante et de la
       Recherche Medicale U314, Centre Hospitalier; Universitaire, Hopital
       Maison Blanche, Reims, France.
 SO    Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):585-90. Unique
       Identifier : AIDSLINE MED/96047083
 AB    The diagnosis of Toxoplasma gondii infection is currently based on
       immunological tests, but tests for IgG and IgM antibodies alone are
       often insufficient to assess the risk of active disease, especially
       during pregnancy and in immunodeficient subjects. The supplementary
       diagnostic value of testing for antitoxoplasmic IgA in cases of acute,
       chronic, congenital and reactivated toxoplasmosis, relative to classical
       immunological tests, was evaluated using two immunocapture tests, one
       based on tachyzoite agglutination and the other on an immunoenzymatic
       complex recognizing the membrane protein P30 of Toxoplasma gondii. A
       total of 4,541 sera from 395 uninfected subjects, 468 immunized subjects
       with chronic infection, 117 subjects with acute infection and 403
       children, 103 of whom had congenital toxoplasmosis, was tested. Specific
       IgA tests were negative in the nonimmune population, but tests for this
       immunoglobulin subtype became positive very rapidly during primary
       infection, and IgA disappeared more rapidly than IgM. In the children
       infected in utero, specific IgA was detected more frequently than IgM.
       In contrast, in a population of HIV-seropositive subjects with clinical
       toxoplasmosis, tests for IgA were poorly sensitive. The two tests for
       specific IgA produced similar results, except in the early stages of
       primary infection, in which immunoenzymatic testing for anti-P30 IgA was
       less sensitive than the agglutination method.
 DE    Agglutination Tests  Animal  Antibodies, Protozoan/*BLOOD  Child  Child,
       Preschool  Female  Human  IgA/*BLOOD  Immunoenzyme Techniques  Infant
       Infant, Newborn  Mice  Pregnancy  Support, Non-U.S. Gov't
       Toxoplasma/*IMMUNOLOGY  Toxoplasmosis/*DIAGNOSIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

