       Document 0650
 DOCN  M9620650
 TI    Polarized Th2 cytokine expression by both mucosal gamma delta and alpha
       beta T cells.
 DT    9602
 AU    Hiroi T; Fujihashi K; McGhee JR; Kiyono H; Department of Oral Biology,
       University of Alabama at Birmingham; Medical Center 35294-2170, USA.
 SO    Eur J Immunol. 1995 Oct;25(10):2743-51. Unique Identifier : AIDSLINE
       MED/96062019
 AB    Currently only limited information is available as to why dominant IgA
       isotype responses are supported by mucosal T cells in effector tissues.
       To address this issue directly, gamma delta and alpha beta T cells were
       isolated from the submandibular gland (SMG) of mice as an example of
       mucosal effector tissues. Freshly isolated CD3+ T cells from this tissue
       contained relatively high numbers of activated cells [approximately 10%
       interleukin-2 receptor (IL-2R)+ cells and 15% of cells in cycle stages S
       and G2 + M], of which 25% and 75% were gamma delta and alpha beta T
       cells, respectively. The cytokine-specific quantitative reverse
       transcriptase-polymerase chain reaction and enzyme-linked immunospot
       analyses revealed that, although both gamma delta and alpha beta T cells
       were capable of producing an array of Th1 or Th2 cytokines following
       stimulation via the T cell receptor-CD3 complex, these mucosal T cells
       were mainly committed to IL-5 and IL-6 expression in vivo (Th2 type).
       Both freshly isolated gamma delta and alpha beta T cells expressed mRNA
       and contained IL-5 and IL-6 spot-forming cells (SFC); however, only the
       latter exhibited high mRNA levels and SFC for a Th1 cytokine
       (interferon-gamma). Taken together, the results show that freshly
       isolated CD3+ T cells from SMG contain activated gamma delta and alpha
       beta T cells which are programmed to produce IL-5 and IL-6. Thus, SMG,
       an example of an IgA effector tissue, can be characterized as a
       Th2-dominant site. However, although both gamma delta and alpha beta T
       cells express cytokine profiles consistent with a Th2 phenotype, only
       the latter subset with a CD4+ CD8- phenotype provided effective help for
       mucosal B cell responses in vitro.
 DE    Animal  Antigens, CD3/IMMUNOLOGY  Base Sequence  Cytokines/*BIOSYNTHESIS
       IgA/BIOSYNTHESIS  Lymphocyte Cooperation  Lymphocyte Transformation
       Mice  Mice, Inbred C3H  Molecular Sequence Data  Polymerase Chain
       Reaction  Receptors, Antigen, T-Cell, alpha-beta/*ANALYSIS  Receptors,
       Antigen, T-Cell, gamma-delta/*ANALYSIS  RNA, Messenger/BIOSYNTHESIS
       Submandibular Gland/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  Th2
       Cells/*IMMUNOLOGY/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

