       Document 0649
 DOCN  M9620649
 TI    Induction of Th1 and Th2 CD4+ T cell responses by oral or parenteral
       immunization with ISCOMS.
 DT    9602
 AU    Maloy KJ; Donachie AM; Mowat AM; Department of Immunology, University of
       Glasgow, Western; Infirmary, Scotland.
 SO    Eur J Immunol. 1995 Oct;25(10):2835-41. Unique Identifier : AIDSLINE
       MED/96062033
 AB    We examined the ability of oral or parenteral immunization with immune
       stimulating complexes containing ovalbumin (ISCOMS-OVA) to prime T cell
       proliferative and cytokine responses. A single subcutaneous immunization
       with ISCOMS-OVA primed potent antigen-specific proliferative responses
       in the draining popliteal lymph node, which were entirely dependent on
       the presence of CD4+ T cells. CD8+ T cells did not proliferate in vitro
       even in the presence of the appropriate peptide epitope and exogenous
       interleukin (IL)-2. Primed popliteal lymph node cells produced IL-2,
       IL-5 and interferon (IFN)-gamma, but not IL-4 when restimulated with OVA
       in vitro. Serum antigen-specific IgG1 and IgG2a antibody responses were
       also primed by subcutaneous immunization with ISCOMS-OVA, confirming the
       stimulation of both Th1 and Th2 cells in vivo. Spleen cells from
       subcutaneously primed mice produced a similar pattern of cytokines,
       indicating that disseminated priming had occurred. Oral immunization
       with ISCOMS-OVA also primed local antigen-specific proliferative
       responses in the mesenteric lymph node and primed an identical pattern
       of systemic cytokine responses in the spleen. The ability of ISCOMS to
       prime both Th1 and Th2 CD4+ T cell responses may be central to their
       potent adjuvant activities and confirm the potential of ISCOMS as future
       oral vaccine vectors.
 DE    Administration, Oral  Animal  CD8-Positive T-Lymphocytes/IMMUNOLOGY
       Female  IgG/BIOSYNTHESIS/IMMUNOLOGY  Immunization/*METHODS  Injections,
       Subcutaneous  ISCOMs/*IMMUNOLOGY  Lymphocyte Cooperation  Lymphocyte
       Transformation  Lymphokines/SECRETION  Mice  Mice, Inbred BALB C  Mice,
       Inbred C57BL  Ovalbumin/IMMUNOLOGY  Spleen/CYTOLOGY/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Th1 Cells/*IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

