       Document 0644
 DOCN  M9620644
 TI    Evidence for Th2 cell-mediated suppression of antibody responses in
       transgenic, beef insulin-tolerant mice.
 DT    9602
 AU    Teng YT; Gorczynski RM; Iwasaki S; Williams DB; Hozumi N; Samuel
       Lunenfeld Research Institute, Mount Sinai Hospital,; Toronto, Canada.
 SO    Eur J Immunol. 1995 Sep;25(9):2522-7. Unique Identifier : AIDSLINE
       MED/96011861
 AB    Clonal deletion, anergy and suppression have all been considered
       mechanisms of immunological tolerance. Although adoptive transfer of
       immunosuppression has been shown to occur in the periphery, particularly
       for transplantation tolerance, it has proven difficult to characterize
       this phenomenon further, due to the lack of suppressor T cell clones. To
       characterize tolerance towards a physiological soluble antigen, we
       constructed beef insulin (BI) transgenic (Tg) BALB/c (H-2d) mice, in
       which the BI transgene is expressed in pancreatic beta cells. These Tg
       mice were tolerant to BI immunization at the level of both humoral and
       cell-mediated immune responses. Adoptive transfer of splenocytes from Tg
       mice into normal syngeneic BALB/c mice demonstrated that the reduction
       in antibody production is regulated by transferred T cells. The cytokine
       profile of T cell clones obtained after selection in vitro demonstrated
       dominant Th1 clones from normal non-Tg mice and dominant Th2 clones from
       Tg mice. Some Th2 clones (CD4+) from Tg mice produced significant
       suppression of antibody production after adoptive transfer into normal
       syngeneic BALB/c mice. These data confirm the existence of Th2
       regulatory T cells in vivo in a model of peripheral tolerance to a
       physiological soluble antigen as a potential mechanism for self
       tolerance.
 DE    Animal  Antibody Formation  Cattle  Clone Cells  Immunotherapy, Adoptive
       Insulin Resistance/*IMMUNOLOGY  Mice  Mice, Inbred BALB C  Mice,
       Transgenic  Spleen/*IMMUNOLOGY  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets/IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

