       Document 0637
 DOCN  M9620637
 TI    Human naive CD4 T cells produce interleukin-4 at priming and acquire a
       Th2 phenotype upon repetitive stimulations in neutral conditions.
 DT    9602
 AU    Demeure CE; Yang LP; Byun DG; Ishihara H; Vezzio N; Delespesse G;
       University of Montreal, Louis-Charles Simard Research Center,;
       Notre-Dame Hospital, Canada.
 SO    Eur J Immunol. 1995 Sep;25(9):2722-5. Unique Identifier : AIDSLINE
       MED/96011894
 AB    The maturation of naive CD4 T cells into interleukin (IL)-4-producing
       effectors was shown to require the presence of IL-4 at priming, the
       cellular origin of which remains unclear. We demonstrate here that naive
       T cells themselves release IL-4 at very low levels that are nevertheless
       sufficient to promote their development into Th2-like cells. This
       conclusion is based on three observations: (1) highly purified human
       naive CD4 T cells, of neonatal or adult origin, develop into Th2
       effectors upon repetitive cycles of stimulation with anti-CD3 monoclonal
       antibody (mAb) cross-linked to CD32-B7 transfected L fibroblasts
       followed by IL-2 expansion; (2) IL-4 protein is readily detectable in
       the concentrated supernatant fluids of priming cultures performed in the
       presence of anti-IL-4 receptor mAb; and (3) addition of anti-IL-4 or
       anti-IL-4 receptor mAb at priming markedly inhibits the acquisition of
       IL-4- and IL-5-producing capacity while enhancing that of
       interferon-gamma.
 DE    Adult  Cell Differentiation/DRUG EFFECTS  Cells, Cultured  CD4-Positive
       T-Lymphocytes/CYTOLOGY/*IMMUNOLOGY  Human
       Interleukin-4/*BIOSYNTHESIS/PHARMACOLOGY  Support, Non-U.S. Gov't  Th2
       Cells/CYTOLOGY/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

