       Document 0615
 DOCN  M9620615
 TI    Human immunodeficiency virus-associated Hodgkin's disease contains
       latent, not replicative, Epstein-Barr virus.
 DT    9602
 AU    Siebert JD; Ambinder RF; Napoli VM; Quintanilla-Martinez L; Banks PM;
       Gulley ML; Department of Pathology, University of Texas Health Science;
       Center, San Antonio 78284-7750, USA.
 SO    Hum Pathol. 1995 Nov;26(11):1191-5. Unique Identifier : AIDSLINE
       MED/96068778
 AB    Severe immunodeficiency is associated with reactivation of latent
       Epstein-Barr virus (EBV) that is manifested by virus replication. It is
       unknown whether EBV replication also occurs in the Hodgkin's disease
       (HD) tissue of patients infected with the human immunodeficiency virus
       (HIV). Therefore, we studied paraffin-embedded lymph nodes from 13 cases
       of HIV-associated HD to determine the latent or replicative state of EBV
       infection. All patients were seropositive HIV-infected men; additional
       clinical information was available for 12 patients. The risk factor(s)
       for HIV infection were homosexuality (n = 7), intravenous drug abuse (n
       = 2), homosexuality and intravenous drug abuse (n = 1), sexual
       promiscuity (n = 1), or hemophilia (n = 1). Advanced clinical stage and
       B symptoms were common at the time of initial diagnosis of HD. The
       histological subtype of Hodgkin's disease was universally mixed
       cellularity, except for a single case classified as nodular sclerosis.
       Seven cases exhibited foci of relative lymphoid depletion. Five cases
       contained foci of necrosis. Reed-Sternberg (RS) cells and RS cell
       variants were positive for CD30/BerH2 and negative for CD45/LCA,
       CD45RO/UCHL1, and CD20/L26 in all cases. Tumor cells were positive for
       CD15/LeuM1 in seven cases. In all 13 cases, RS cells and RS cell
       variants were infected by latent EBV as shown by in situ hybridization
       to EBV-encoded ribonucleic acid (EBER1). In 12 of 13 cases neoplastic
       cells coexpressed EBV latent membrane protein 1 (LMP1). EBV replication
       was examined by two different methods: immunohistochemistry to identify
       EBV-encoded BZLF1 protein and in situ hybridization to detect EBV BHLF1
       transcripts. No positivity in RS or RS cell variants was detected with
       either assay of EBV replication (95% confidence interval [CI] = 0% to
       23%). The findings confirm that EBV is detected more frequently in
       HIV-associated HD when compared with immunocompetent patients with HD.
       The findings also suggest that EBV is tightly latent within RS and RS
       cell variants of HIV-associated HD. It appears that factors other than
       host immune status are important in maintaining EBV latency in
       HIV-associated HD.
 DE    Adult  Antigens, CD30/ANALYSIS  DNA-Binding
       Proteins/ANALYSIS/GENETICS/METABOLISM  Herpesvirus 4,
       Human/*GENETICS/ISOLATION & PURIF/PHYSIOLOGY  Hodgkin's
       Disease/*COMPLICATIONS/PATHOLOGY/*VIROLOGY  Human  HIV
       Infections/*COMPLICATIONS/PATHOLOGY  Immunohistochemistry
       Immunophenotyping  In Situ Hybridization  Male  Middle Age
       Reed-Sternberg Cells/CHEMISTRY/IMMUNOLOGY/PATHOLOGY  RNA,
       Messenger/ANALYSIS/GENETICS  RNA, Viral/ANALYSIS/GENETICS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't,
       P.H.S.  Trans-Activators/ANALYSIS/GENETICS/METABOLISM  Viral Matrix
       Proteins/ANALYSIS/GENETICS/METABOLISM  Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

