       Document 0608
 DOCN  M9620608
 TI    Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein
       120 generated by repeated immunization with glycoprotein 120 from a
       single isolate, or by sequential immunization with glycoprotein 120 from
       three isolates.
 DT    9602
 AU    Reeves JP; Lo CY; Klinman DM; Epstein SL; Laboratory of Molecular
       Immunology, Food and Drug Administration,; Bethesda, Maryland 20892,
       USA.
 SO    Hybridoma. 1995 Jun;14(3):235-42. Unique Identifier : AIDSLINE
       MED/96082393
 AB    Mouse hybridomas were isolated that produce monoclonal antibodies (MAbs)
       to several regions of the HIV envelope, gp120, which may be used to map
       immunogenic regions in animal and human immunity. One series of MAbs was
       generated by repeated immunization with recombinant gp120 (rgp120) from
       a single isolate, and a second series by sequential immunization with
       rgp120 from three isolates. All MAbs bound to rgp120IIIB, but only one
       bound well to cell surface-expressed gp160. Synthetic peptides spanning
       much of the length of gp120 were used to map MAb reactivity. Two MAbs
       were mapped to the C1 region, one to the C2 region, and three to the C5
       region by this approach. Six distinct epitopes were detected by
       competitive binding analysis. One MAb binding the C1 region blocks
       binding of CD4 to rgp120IIIB, binds by ELISA to rgp120MN (an isolate not
       used during immunization), and binds to cell surface-expressed gp160.
       These hybridomas will be made available to the scientific community
       through a hybridoma culture facility.
 DE    Animal  Antibodies, Monoclonal/*BIOSYNTHESIS/CHEMISTRY  Antigens,
       CD4/IMMUNOLOGY/METABOLISM  AIDS Vaccines/*IMMUNOLOGY  Binding Sites,
       Antibody  Cell Fusion/IMMUNOLOGY  Cross Reactions  Enzyme-Linked
       Immunosorbent Assay  Female  Fluorescent Antibody Technique  Gene
       Products, env/ANALYSIS  Human  HIV Envelope Protein gp120/*IMMUNOLOGY
       HIV-1/*ISOLATION & PURIF  Mice  Mice, Inbred BALB C  Protein
       Precursors/ANALYSIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

