       Document 0602
 DOCN  M9620602
 TI    Th1 cells specific for HIV-1 gag p24 are less efficient than Th0 cells
       in supporting HIV replication, and inhibit virus replication in Th0
       cells.
 DT    9602
 AU    Vyakarnam A; Matear PM; Martin SJ; Wagstaff M; Department of Immunology,
       University College London Medical; School, UK.
 SO    Immunology. 1995 Sep;86(1):85-96. Unique Identifier : AIDSLINE
       MED/96091076
 AB    This report provides three lines of evidence to suggest that T-helper
       type 1 (Th1) and type 0 (Th0) cells could play an opposing role in
       acquired immune deficiency syndrome (AIDS). Using a panel of Th1 and Th0
       clones specific for human immunodeficiency virus-1 (HIV-1) gag p24,
       derived from seronegative volunteers immunized with gag p24: Ty
       virus-like particles, a Th1 clone specific for tuberculin (PPD), and a
       Th0 clone derived by random activation from the same volunteer, we have
       demonstrated the following differences in the capacity of these clones
       to regulate the in vitro replication of HIV. (1) Th1 clones were less
       efficient than Th0 clones in supporting HIV replication, both in their
       resting state (by 10-1000-fold) and after antigen activation (by five to
       100-fold). Furthermore, the infectious titre of HIV recovered from the
       Th0 population was more than 1000-fold higher than virus from the Th1
       population, and the number of HIV-infected Th0 cells was five to 16
       times higher than the number of infected Th1 cells. (2) Antigen- or
       mitogen-activated Th1, but not Th0 clones, inhibited HIV in bystander
       CEM-4 cells. Th1 cells also inhibited HIV in autologous and allogeneic
       Th0 cells. The level of inhibition in these experiments ranged from 50%
       to 100% and was three to 10-fold higher and more sustained in the
       presence of p24-specific clones compared to the PPD-specific Th1 clone.
       The capacity of Th1 cells to inhibit HIV in neighbouring cells was also
       reflected in the reduced replication of HIV in the clones immediately
       after antigen activation compared to unstimulated cells. Kinetic studies
       of virus production, cytokine release and proliferation showed that
       inhibition of HIV was associated with peak cytokine release and
       preceeded proliferation. (3) The Th1 clones had higher cytolytic
       potential than the Th0 clones. Therefore, the HIV inhibitory activity of
       Th1 cells could be partly due to cell to cell killing. These data
       demonstrate the opposing effects of Th1 and Th0 cells on the in vitro
       replication of HIV, and suggest that Th1 cells might be important in
       immunity whereas Th0/Th2 cells might lay a role in promoting disease.
 DE    Clone Cells  CD4-Positive T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  Human  HIV
       Core Protein p24/*IMMUNOLOGY  HIV-1/IMMUNOLOGY/*PHYSIOLOGY  Interferon
       Type II/METABOLISM  Interleukin-2/METABOLISM  Interleukin-4/METABOLISM
       Lymphocyte Transformation  Support, Non-U.S. Gov't  Th1
       Cells/*IMMUNOLOGY/VIROLOGY  Virus Replication/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

