       Document 0597
 DOCN  M9620597
 TI    Beta 2-microglobulin-deficient mice demonstrate class II MHC restricted
       anti-viral CD4+ but not CD8+ CTL against influenza-sensitized autologous
       splenocytes.
 DT    9602
 AU    Taylor SF; Bender BS; Department of Veterans Affairs Medical Center,
       University of; Florida College of Medicine, Gainesville 32608, USA.
 SO    Immunol Lett. 1995 May;46(1-2):67-73. Unique Identifier : AIDSLINE
       MED/96078156
 AB    Mice transgenic for beta 2-microglobulin gene deletion (beta 2M-/-) can
       clear respiratory pathogens but at a slower rate than control mice. How
       these mice eliminate virus is not known, but the process is believed to
       involve CD4+ T cells. Recent studies from other laboratories have
       suggested a role for CD8+ cytotoxic T lymphocytes (CTLs) in both
       recognition of beta 2M deficient cells by allogeneic mice and rejection
       of MHC-incompatible tumor cells by beta 2M-/- mice. After influenza
       inoculation, we found no evidence for anti-influenza CD8 CTL activity
       from the lungs or spleens of beta 2M-/- mice. Anti-influenza CD4+,
       class-II restricted CTL activity was demonstrated from both the lungs
       and spleens. We next used mitogen-stimulated splenocytes from beta 2M-/-
       mice for targets in an in vitro CTL assay. This method for determining
       MHC class II CTL activity showed that the lungs and spleens of
       influenza-infected beta 2M-/- mice contained precursors to CD4+, but not
       CD8+, effector CTLs. The data indicate that CD8+ CTLs have no role in
       anti-viral activity in beta 2M-/- mice. Development of anti-tumor CTLs
       and anti-viral CTLs may arise via different mechanisms.
 DE    beta 2-Microglobulin/*DEFICIENCY/GENETICS  Animal  Cytotoxicity,
       Immunologic  CD4-Positive T-Lymphocytes/IMMUNOLOGY/VIROLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY/VIROLOGY  Female
       Histocompatibility Antigens Class II/*IMMUNOLOGY/METABOLISM
       Immunophenotyping  Lung/CYTOLOGY  Mice  Mice, Transgenic
       Orthomyxoviridae/*IMMUNOLOGY  Spleen/CYTOLOGY/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  T-Lymphocytes,
       Cytotoxic/IMMUNOLOGY/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

