       Document 0581
 DOCN  M9620581
 TI    Viral activation from latency during retrodifferentiation of U937 cells
       exposed to phorbol ester followed by infection with human
       immunodeficiency virus type 1.
 DT    9602
 AU    Okada Y; Kimura T; Kameoka M; Kishi M; Azuma I; Ikuta K; Institute of
       Immunological Science, Hokkaido University, Sapporo,; Japan.
 SO    Immunopharmacology. 1995 Jun;30(1):27-39. Unique Identifier : AIDSLINE
       MED/96078206
 AB    To determine the mechanism underlying the human immunodeficiency virus
       type 1 (HIV-1) latency and its activation in monocyte/macrophage
       lineage, the human promonocytic cell line U937 was infected with HIV-1
       after differentiation with varied doses of phorbol 12-myristate
       13-acetate (PMA). Variously differentiated intermediate stages were
       generated in U937 cells in a dose-dependent manner. When these cells
       were infected with lymphotropic HIV-1, the kinetics of the production of
       HIV-1 DNA, the appearance of HIV-1 antigen-positive cells, and viral
       production in the conditioned media were slower at higher doses of PMA.
       This different susceptibility to the infection was not due to the rate
       of HIV-1 adsorption. Viral replication from latency in the
       differentiated cells was activated in proportion with the
       retrodifferentiation observed in long-term cultures of the host cells.
       Thus, our data demonstrate the close correlation between the regulation
       of HIV-1 replication and the differentiation stage of
       monocyte/macrophage lineage cells at the time of HIV-1 infection. The
       retrodifferentiation phenomenon in infected cells seems to be
       particularly important for understanding the mechanisms for HIV-1
       activation from latency.
 DE    Antigens, CD4/DRUG EFFECTS  Base Sequence  Cell Differentiation/DRUG
       EFFECTS/IMMUNOLOGY  Dose-Response Relationship, Drug  Human
       HIV-1/*GROWTH & DEVELOPMENT/IMMUNOLOGY  Immunity, Natural  Leukemia,
       Monocytic, Acute/IMMUNOLOGY/VIROLOGY  Molecular Sequence Data
       Monocytes/IMMUNOLOGY/VIROLOGY  Support, Non-U.S. Gov't
       Tetradecanoylphorbol Acetate/*PHARMACOLOGY  Tumor Cells, Cultured  Virus
       Activation/DRUG EFFECTS/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

