       Document 0580
 DOCN  M9620580
 TI    High susceptibility of U937-derived subclones to infection with human
       immunodeficiency virus type 1 is correlated with virus-induced cell
       differentiation and superoxide generation.
 DT    9602
 AU    Kameoka M; Kimura T; Okada Y; Nakaya T; Kishi M; Ikuta K; Institute of
       Immunological Science, Hokkaido University, Sapporo,; Japan.
 SO    Immunopharmacology. 1995 Jun;30(1):89-101. Unique Identifier : AIDSLINE
       MED/96078212
 AB    The promonocytic human leukemic cell line U937, when infected with
       lymphotropic human immunodeficiency virus type 1 (HIV-1), becomes a
       continuous virus producer. A total of 46 U937-derived subclones in
       suspension was isolated and classified into three (2 high, 42 middle,
       and 2 low) types based on their susceptibility to the infection. By
       analyzing subclones before infection, we found that the high-type
       subclones expressed LFA-1 antigens at a relatively low level. In
       addition, the ability of these subclones to induce adherence after
       exposure to phorbol 12-myristate 13-acetate (PMA) was reduced. In
       contrast, a transition by HIV-1 infection to adherent macrophage-like
       cells was induced only in the high-type, but not in the low-type
       subclones. The high-type adherent cells obtained by HIV-1 infection were
       followed by further lineage to become retrodifferentiated suspension
       cells showing reduced syncytia formation ability. Superoxide was
       generated in the high-type subclones, without PMA-mediated
       differentiation, from the early stage of infection before HIV-1
       replication, as well as during undifferentiated, differentiated and
       retrodifferentiated stages. In contrast, it was only transiently
       generated at acute phase of HIV-1 replication in low-type subclones.
       Long-term culture of the low-type subclones decreased the expression of
       major structural viral protein Gag and also virus production. Thus, the
       mechanism by which PMA differentiates U937 cells is not the same as that
       induced by HIV-1 infection. The latter mechanism results in high
       susceptibility to infection. The HIV-1 phenotypes of finally obtained
       persistently infected cells were also affected by the cell stages at the
       time of infection.
 DE    Cell Cycle  Cell Differentiation/IMMUNOLOGY  Clone
       Cells/METABOLISM/PATHOLOGY/VIROLOGY  Human  HIV
       Infections/METABOLISM/*PATHOLOGY/VIROLOGY  Interferon Type II/PHYSIOLOGY
       Leukemia, Monocytic, Acute/METABOLISM/*PATHOLOGY/VIROLOGY
       Superoxides/*METABOLISM  Support, Non-U.S. Gov't  Tumor Cells, Cultured
       Tumor Necrosis Factor/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

