       Document 0577
 DOCN  M9620577
 TI    Cytotoxic activity of CD4+ T cells against autologous tumor cells.
 DT    9602
 AU    Konomi Y; Sekine T; Takayama T; Fuji M; Tanaka T; RI Laboratory,
       National Cancer Center Research Institute, Tokyo.
 SO    Jpn J Cancer Res. 1995 Sep;86(9):854-60. Unique Identifier : AIDSLINE
       MED/96042670
 AB    The 51Cr-release assay is mostly applied to detecting the cytotoxic
       activity of CD8+ T cells, and little is known about the activity of CD4+
       T cells. Therefore, the correlation between the cytotoxic activity of
       CD4+ or CD8+ T cells and the incubation period with autologous tumor
       cells was analyzed by two methods. The incubation periods were 4 and 20
       h (4 h and 20 h assay) for the 51Cr-release assay. Eight pairs of tumor
       cells and T cells were assayed. T cells were fractionated into CD4+ and
       CD8+ T cells by using magnetic beads and panning methods, and those
       cells were activated by culture with recombinant interleukin-2 and
       immobilized anti-CD3 monoclonal antibody. In 6 out of 8 cases, no
       cytotoxic activity of CD4+ T cells was detected by the 4 h assay,
       whereas cytotoxic activity was detected in all cases in the 20 h assay.
       The cytotoxic activities in 20 h assay of CD4+ T cells were increased
       67-fold in comparison with the activities in 4 h assay (range: 5-197).
       In the case of CD8+ T cells, cytotoxic activities were detected in 6 out
       of 8 cases in the 4 h assay. The lytic unit ratio of CD4+ and CD8+ T
       cells was calculated as 1.5 in the 20 h assay (range: 0.2- > 7.2) versus
       0.4 in the 4 h assay (range: < 0.1-1.3). Cytotoxic activities in
       colorimetric assay using Crystal Violet with a 24 h incubation were
       similar to those in the 20 h 51Cr-release assay in all eight cases.
       These results indicate that CD4+ T cells have cytotoxic activity as
       strong as that of CD8+ T cells towards autologous tumor cells.
 DE    Adult  Aged  Aged, 80 and over  Chromium/PHARMACOKINETICS  Chromium
       Radioisotopes  Colorimetry  Comparative Study  Cytotoxicity, Immunologic
       CD4-CD8 Ratio  CD4-Positive T-Lymphocytes/CYTOLOGY/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/CYTOLOGY/IMMUNOLOGY  Female  Gentian Violet
       Human  *Immunotherapy, Adoptive  Lymphocytes,
       Tumor-Infiltrating/CYTOLOGY/IMMUNOLOGY  Male  Middle Age
       Neoplasms/IMMUNOLOGY/METABOLISM/PATHOLOGY/*THERAPY  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

