       Document 0526
 DOCN  M9620526
 TI    CD8highCD57+ T lymphocytes in normal, healthy individuals are
       oligoclonal and respond to human cytomegalovirus.
 DT    9602
 AU    Wang EC; Moss PA; Frodsham P; Lehner PJ; Bell JI; Borysiewicz LK;
       Department of Medicine, University of Wales College of Medicine,; Health
       Hospital, Cardiff, United Kingdom.
 SO    J Immunol. 1995 Nov 15;155(10):5046-56. Unique Identifier : AIDSLINE
       GENBANK/Z50839
 AB    CD8+CD57+ lymphocytes from normal peripheral blood are divided into T
       cells expressing high levels of CD8 and NK cells expressing low levels
       of surface CD8. Increased numbers of CD8highCD57+ T cells correlate with
       previous exposure to human cytomegalovirus (HCMV) infection, but no
       virus-specific function or function for CD8highCD57+ cells has been
       recorded. We have studied the TCR repertoire and responses of the
       CD8highCD57+ population induced by virus-infected fibroblasts in healthy
       individuals. Using three-color flow cytometry of PBL, we detected
       restricted TCRBV usage in the CD8highCD57+ subset of 11/15 subjects,
       compared with 1/15 in the CD8+, CD57- subset. The results of anchored
       PCR and sequencing also showed oligoclonality of TCR; 40-70% of
       CD8highCD57+ lymphocytes (10-20% of total CD8+ T cells) were derived
       from single clones. Such expansions were stable with time and detected
       in one subject over a 2-yr period. Functionally, CD8highCD57+
       lymphocytes proliferated strongly to HCMV-, but not HSV-, VZV-, or
       influenza-infected fibroblasts in an MHC-unrestricted manner in vitro,
       including preferential augmentation of particular in vivo oligoclonally
       expanded subpopulations. HCMV-specific MHC-restricted CTL were detected,
       but limiting dilution analysis showed that these were a minority (< 10%)
       and not the oligoclonal subsets. In contrast, depletion of oligoclonally
       expanded CD8highCD57+ subpopulations, resulted in the increase of
       HCMV-specific CTL, suggesting functional heterogeneity in these cells.
 DE    Adult  Amino Acid Sequence  Antigens, CD57/*IMMUNOLOGY  Base Sequence
       Cells, Cultured  Clone Cells  Cytomegalovirus/*IMMUNOLOGY
       Cytomegalovirus Infections/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/CYTOLOGY/*IMMUNOLOGY/*VIROLOGY  Human  Middle Age
       Molecular Sequence Data  Receptors, Antigen, T-Cell/GENETICS/*IMMUNOLOGY
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

