       Document 0515
 DOCN  M9620515
 TI    Host cell-derived complement control proteins CD55 and CD59 are
       incorporated into the virions of two unrelated enveloped viruses. Human
       T cell leukemia/lymphoma virus type I (HTLV-I) and human cytomegalovirus
       (HCMV).
 DT    9602
 AU    Spear GT; Lurain NS; Parker CJ; Ghassemi M; Payne GH; Saifuddin M;
       Department of Immunology/Microbiology, Rush Presbyterian-St.; Luke's
       Medical Center, Chicago, IL 60612, USA.
 SO    J Immunol. 1995 Nov 1;155(9):4376-81. Unique Identifier : AIDSLINE
       MED/96025897
 AB    The current study was undertaken to determine whether the human T cell
       leukemia/lymphoma oncovirus type I (HTLV-I) and the herpesvirus human
       cytomegalovirus (HCM) incorporate host cell-derived C regulatory
       proteins. Our experiments showed that both CD59 and CD55 were associated
       with the external membrane of HTLV-I derived from MT2 cells, since virus
       could be captured by mAbs to these proteins, and antisera to CD55 and
       CD59 induced C-mediated lysis of HTLV-I virions. Additionally, both CD55
       and CD59 were detected by immunoblot analysis of purified HTLV-I.
       Purified HCMV produced in human foreskin fibroblasts (HFF) also
       contained both CD55 and CD59, as detected by immunoblot analysis.
       However, treatment with anti-CD55, but not anti-CD59, reduced the HCMV
       infectious titer in the presence of C. Additional studies determined
       whether HTLV-I-associated CD55 and CD59 participated in the resistance
       of the virus to C-mediated lysis. Treatment of virus with
       phosphatidylinositol-specific phospholipase C (PI-PLC), which removes
       glycosylphosphatidylinositol-anchored CD55 and CD59, increased the
       sensitivity of HTLV-I to C-mediated destruction in the presence of
       anti-HTLV-I Abs. Reconstitution of PI-PLC-treated virus with purified
       CD55 and CD59 restored resistance to C. These experiments show that
       HTLV-I and HCMV acquire C control proteins from host cells. Together
       with our previous experiments showing that both CD55 and CD59 are
       present on HIV-1, these studies demonstrate a mechanism by which a
       variety of enveloped viruses may acquire resistance to C-mediated
       destruction.
 DE    Antigens, CD55/ANALYSIS/*METABOLISM/PHYSIOLOGY  Antigens,
       CD59/ANALYSIS/*METABOLISM/PHYSIOLOGY  Cell Line  Complement
       Inactivators/ANALYSIS/*IMMUNOLOGY/METABOLISM
       Cytomegalovirus/*IMMUNOLOGY/METABOLISM  Human
       HTLV-I/*IMMUNOLOGY/METABOLISM  Support, U.S. Gov't, P.H.S.  Viral
       Envelope Proteins/ANALYSIS/*IMMUNOLOGY/METABOLISM
       Virion/IMMUNOLOGY/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

